Molecular Cloning of the Transglutaminase Gene from Bacillus subtilis and Its Expression in Escherichia coli
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概要
- 論文の詳細を見る
We cloned and characterized a gene, tgl, encoding transglutaminase in Bacillus subtilis. The tgl gene contained a open reading frame 735-nucleotides long that encoded a 245-residue protein with the molecular weight of 28,300. The deduced amino acid sequence had little sequence similarity with sequences of other transglutaminases from a Streptoverticillium sp. or from mammals. The -10 and -35 regions of a putative promoter resembled the consensus sequence for the σ^K-dependent promoter. In addition, a sequence similar to the consensus sequence for the GerE binding site was found upstream from this region. These findings suggested that tgl was transcribed in the mother cells during a late stage of sporulation. Evidence for this suggestion was that transglutaminase activity was detected in sporulating cells during the same stage. Trans glutaminase activity was detected in Escherichia coli cells transformed with a plasmid for expression of the tgl gene.
- 社団法人日本農芸化学会の論文
- 1998-06-23
著者
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Hashiguchi Kenichi
Central Research Laboratories Ajinomoto Co. Inc.
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KOBAYASHI Katsunori
Central Research Laboratories, Ajinomoto Co., Inc.,
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YOKOZEKI Kenzo
Central Research Laboratories, Ajinomoto Co., Inc.,
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YAMANAKA Shigeru
Central Research Laboratories, Ajinomoto Co., Inc.,
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Yokozeki K
Ajinomoto. Co. Inc. Kawasaki Jpn
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Yokozeki Kenzo
Aminoscience Laboratories Ajinomoto. Co. Inc.
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Yamanaka Shigeru
Central Research Laboratories Ajinomoto Co. Inc.
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Yamanaka Shigeru
Faculty Of Txtile Science And Technology Shishu University
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Hashiguchi Ken-ichi
Central Research Laboratories Ajinomoto Co. Inc.
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Kobayashi Katsunori
Central Research Laboratories Ajinomoto Co. Inc.
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Kubota Koji
The Research Institute Of Electrical Communication Tohoku University Presently The Technology Develo
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Kobayashi K
Department Of Life Science (sports Sciences) The University Of Tokyo
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Yokozeki Kenzo
Central Research Laboratories Of Ajinomoto Co.
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Yamanaka S
Faculty Of Textile Science And Technology Shinshu University
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Yamanaka Shigeru
Microbiology Group Institute Of Life Sciences Ajinomoto Co. Inc.:experimental Farm Faculty Of Textil
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Kubota K
The Research Institute Of Electrical Communication Tohoku University Presently The Technology Develo
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Yamanaka Shigeru
Basic Research Laboratories Central Research Laboratories Ajinomoto Co. Inc.
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