Subsite Structure and Catalytic Mechanism of a New Glycosyltrehaloseproducing Enzyme isolated from the Hyperthermophilic Archaeum, Sulfolobus solfataricus KM1
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概要
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A glycosyltrehalose-producing enzyme from Sulfolobus solfataricus KM1 catalyzes a conversion of maltooligosaccharides to glycosyltrehaloses and also hydrolyzes maltooligosaccharides to liberate glucose, as a side reaction. From the sum of the conversion and hydrolysis reaction rates, the rate parameters involved in the"splitting"of the α-1,4 glucosidic linkage were calculated. From the data obtained, the subsite structure for maltooligosaccharides was identified. From the analysis of the hydrolysate of maltotriose in [^<18>O] labeled H_2O, the hypothesis of the C1-O bond splitting and the formation of a glycosyl (maltosyl)-enzyme intermediate was strongly supported. From the analysis of the reaction product in the presence of [^3H] labeled glucose, the occurrence of intermolecular transglycosylation was confirmed. These data strongly support the suggestion that the catalytic mechanism of this enzyme is a transglycosylation.
- 社団法人日本農芸化学会の論文
- 2000-02-23
著者
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Kato M
Nagoya Univ. Aichi Jpn
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KATO Masaru
Applied Bioresearch Center, Kirin Brewery Co., Ltd.
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KETTOKU Masako
Applied Bioresearch Center, Kirin Brewery Co., Ltd.
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Kobayashi K
Inst. Physical And Chemical Res.(riken) Saitama Jpn
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KOBAYASHI Kazuo
Central Laboratory, Kirin Brewery Co.
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Kato M
Dep. Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya Univ.
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TAKEHARA Kyoko
Applied Bioresearch Center Kirin Brewery Co., Ltd.
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SHIMIZU Toshiyuki
Pharmaceutical Research Laboratories Kirin Brewery Co., Ltd.
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Kobayashi K
Department Of Life Science (sports Sciences) The University Of Tokyo
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Takehara Kyoko
Applied Bioresearch Center Kirin Brewery Co. Ltd.
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Shimizu Toshiyuki
Pharmaceutical Research Laboratories Kirin Brewery Co. Ltd.
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Kobayashi Kazuo
Central Laboratories For Key Technology Kirin Brewery Co. Ltd.
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