Mechanism of Purine Arabinoside Synthesis by Bacterial Transarabinosylation Reaction
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概要
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The mechanism of purine arabinoside synthesis from uracil arabinoside and purine bases via the bacterial transarabinosylation reaction was investigated. Arabinose-1-phosphate was isolated from the reaction mixture in the form of the barium salt and proved to be the intermediate of the reaction. Two enzyme fractions were obtained from Enterobacter aerogenes by means of heat treatment, ammonium sulfate fractionation and DEAE-cellulose column chromatography. One enzyme split uracil arabinoside into uracil and arabinose-1-phosphate in the presence of inorganic phosphate and the other synthesized hypoxanthine arabinoside from arabinose-1-phosphate and hypoxanthine. The substrate specificity of these enzymes indicated that the former was uridine phosphorylase and the latter was purine nucleoside phosphorylase, respectively. Hypoxanthine arabinoside was synthesized from uracil arabinoside and hypoxanthine only in the presence of both enzymes and inorganic phosphate.
- 社団法人 日本農芸化学会の論文
著者
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Yamanaka Shigeru
Central Research Laboratories Ajinomoto Co. Inc.
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Yamazaki Akihiro
Central Research Laboratories Ajionmoto Co. Inc.
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Hirose Yoshio
Central Research Laboratories Of Ajinomoto Co. Inc.
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Yoshinaga Fumihiro
Central Research Laboratories Of Ajinomoto Co. Inc.
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YAMAZAKI Akihiro
Central Research Laboratories, Ajinomoto Co., Inc.
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UTAGAWA Takashi
Central Research Laboratories of Ajinomoto Co., Inc.
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MORISAWA Hirokazu
Central Research Laboratories of Ajinomoto Co., Inc.
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UTAGAWA Takashi
Central Research Laboratories, Ajinomoto Co., Inc.
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YOSHINAGA Fumihiro
Central Research Laboratories, Ajinomoto Co., Inc.
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HIROSE Yoshio
Central Research Laboratories, Ajinomoto Co., Inc.
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HIROSE Yoshio
Central Research Laboratories of Ajinomoto Co.
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