Properties of Nucleoside Phosphorylase from Enterobacter aerogenes

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概要

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• The properties of uridine phosphorylase (UPase) and purine nucleoside phosphorylase (PNPase) at high temperature were investigated. Both enzymes were found to be distributed in a wide range of bacteria and were partially purified from Enterobacter aerogenes AJ 11125 by heat treatment, ammonium sulfate fractionation and column chromatographies onDEAE-cellulose and Sephadex G-150. The UPase Was purified 109-fold, and it showed an optimum pH of 8.5 and optimum temperature of 65°C, and activity toward uridine, 2-deoxyuridine, thymidine and uracil arabinoside but not cytidine. The Km values of UPase for uridine were 0.7 mm at 40°C and 1.8mM at 60°C. The PNPase was purified 83-fold, and it showed an optimum pH of 6.8 and optimum temperature of 60°C, and significant activity toward purine arabinosides as well as purine ribosides. The Km values of PNPase for inosine were 0.8 mM at 40°C and 2.2 mM at 60°C.

• 社団法人 日本農芸化学会の論文

著者

• Yamanaka Shigeru

  Central Research Laboratories Ajinomoto Co. Inc.

• Yamazaki Akihiro

  Central Research Laboratories Ajionmoto Co. Inc.

• Hirose Yoshio

  Central Research Laboratories Of Ajinomoto Co. Inc.

• Yoshinaga Fumihiro

  Central Research Laboratories Of Ajinomoto Co. Inc.

• UTAGAWA Takashi

  Central Research Laboratories of Ajinomoto Co., Inc.

• MORISAWA Hirokazu

  Central Research Laboratories of Ajinomoto Co., Inc.

• HIROSE Yoshio

  Central Research Laboratories of Ajinomoto Co.

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