Protease Catalyzed Reaction of Polyamine Incorporation into Protein

スポンサーリンク

概要

• 論文の詳細を見る

• Radiolabeled putrescine incorporation into dimethyl casein is widely measured in transglutaminase assays. We assessed the hypothesis that proteases can catalyze this reaction. Acceleration of putrescine incorporation by chymotrypsin, subtilisin, and papain, all purified to homogeneity, was investigated, and all of the samples tested accelerated the reaction. These effects disappeared when the proteases were first boiled. The rate of incorporation was proportional to the enzyme concentration. The amount incorporated when chymotrypsin was tested was proportional to the reaction time. On the basis of these findins, we concluded that some proteases catalyze reactions with putrescine incorporation.

• 社団法人日本農芸化学会の論文
• 1997-09-23

著者

• KOBAYASHI Katsunori

  Central Research Laboratories, Ajinomoto Co., Inc.,

• YOKOZEKI Kenzo

  Central Research Laboratories, Ajinomoto Co., Inc.,

• YAMANAKA Shigeru

  Central Research Laboratories, Ajinomoto Co., Inc.,

• Suzuki S

  Aminoscience Laboratories Ajinomoto Co. Inc.

• Yokozeki K

  Ajinomoto. Co. Inc. Kawasaki Jpn

• Yokozeki Kenzo

  Aminoscience Laboratories Ajinomoto. Co. Inc.

• Suzuki Shunichi

  Central Research Laboratories Ajinomoto Co. Inc.

• Yamanaka Shigeru

  Central Research Laboratories Ajinomoto Co. Inc.

• Yamanaka Shigeru

  Faculty Of Txtile Science And Technology Shishu University

• Kobayashi Katsunori

  Central Research Laboratories Ajinomoto Co. Inc.

• Kubota Koji

  The Research Institute Of Electrical Communication Tohoku University Presently The Technology Develo

• Kobayashi K

  Department Of Life Science (sports Sciences) The University Of Tokyo

• Yokozeki Kenzo

  Central Research Laboratories Of Ajinomoto Co.

• Yamanaka S

  Faculty Of Textile Science And Technology Shinshu University

• Yamanaka Shigeru

  Microbiology Group Institute Of Life Sciences Ajinomoto Co. Inc.:experimental Farm Faculty Of Textil

• Kubota K

  The Research Institute Of Electrical Communication Tohoku University Presently The Technology Develo

• Yamanaka Shigeru

  Basic Research Laboratories Central Research Laboratories Ajinomoto Co. Inc.

関連論文

• Preparation and Characterization of Ca^-free Methanol Dehydrogenase from Hyphomicrobium denitrificans A3151
• 2P149 A novel supramolecular assembly and intermolecular electrontransfer process of Hyphomicrobium Cu-containing nitrite reductase(33. Redox enzyme,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
• Structural Reorganization of the Copper Binding Site Involving Thr^ of Mavicyanin from Cucurbita pepo medullosa (Zucchini) upon Reduction
• Cytochrome c_ Is a Mediator of Electron Transfer between Copper-Containing Nitrite Reductase and Azurin in a Denitrifying Bacterium, Achromobacter xylosoxidans
• Pulse Radiolysis of Hexameric Nitrite Reductase Containing Two Type 1 Cu Sites in a Monomer
• 1P-060 銅型亜硝酸還元酵素-基質複合体結晶を用いたX線照射による効果の結晶学的研究(蛋白質・構造機能相関(1),第46回日本生物物理学会年会)
• 1P-059 銅型亜硝酸還元酵素とシトクロムcの電子伝達複合体相互作用界面における部位特異的変異導入の効果(蛋白質・構造機能相関(1),第46回日本生物物理学会年会)
• Identification of a Blue Copper Protein from Hyphomicrobium denitrificans and its Functions in the Periplasm
• Molecular Cloning of the Transglutaminase Gene from Bacillus subtilis and Its Expression in Escherichia coli
• The Intramolecular Electron Transfer between the Type 1 Cu and the Type 2 Cu in a Mutant of Hyphomicrobium Nitrite Reductase
• Effect of Arginine at Type 2 Cu Site on Spectroscopic Features and Enzymatic Activity of Copper-containing Nitrite Reductase
• The pH-Dependent Changes of Intramolecular Electron Transfer on Copper-Containing Nitrite Reductase
• Type1 Cu Structure of Blue Nitrite Reductase from Alcaligenes xylosoxidans GIFU 1051 at 2.05 Å Resolution: Comparison of Blue and Green Nitrite Reductases
• Characterization of Azide-Binding Type 2 Cu(II) Site of Nitrite Reductase
• Isolation and Characterization of Two Distinct Azurins from Alcaligenes xylosoxidans subsp.xylosoxidans NCIB11015 or GIFU1051
• Low Power Current-Cut Switched-Current Matched Filter for CDMA (Special Issue on Low-Power High-Performance VLSI Processors and Technologies)
• Novel Low-Power Switched-Current Matched Filter for Direct-Sequence Code-Division-Multiple-Access Wireless Communication
• Cystothiazoles A and B, New Bithiazole-type Antibiotics from the Myxobacterium Cystobacter fuscus
• Gene Cloning and Expression of New Trehalose-producing Enzymes from the Hyperthermophilic Archaeum Sulfolobus solfataricus KM1
• Gene Analysis of Trehalose-producing Enzymes from Hyperthermophilic Archaea in Sulfolobales
• Reaction Mechanism of a New Glycosyltrehalose-hydrolyzing Enzyme Isolated from the Hyperthermophilic Archaeum, Sulfolobus solfataricus KM1
• Reaction Mechanism of a New Glycosyltrehalose-producing Enzyme Isolated from the Hyperthermophilic Archaeum, Sulfolobus solfataricus KM1
• Purification and Characterization of New Trehalose-producing Enzymes Isolated from the Hyperthermophilic Archae, Sulfolobus solfataricus KM1
• Optimal Conditions for the Enzymatic Production of L-Aromatic Amino Acids from the Corresponding 5-Substituted Hydantoins(Microbiology & Fermentation Industry)
• Optimal Conditions for the Enzymatic Production of D-Amino Acids from the Corresponding 5-Substituted Hydantoins(Microbiology & Fermentation Industry)
• Enzymatic Production of L-Tryptophan from DL-5-Indolylmethylhydantoin by Mutants of Flavobacterium sp. T-523(Microbiology & Fermentation Industry)
• Screening of Microorganisms Producing D-p-Hydroxyphenyl-glycine from DL-5-(p-Hydroxyphenyl)hydantoin(Microbiology & Fermentation Industry)
• Coenzyme Specificity of Enzymes in the Oxidative Pentose Phosphate Pathway of Gluconobacter oxydans(Microbiology & Fermentation Technology)
• Construction of a Vector Plasmid for Use in Gluconobacter oxydans(Microbiology & Fermentation Technology)
• Functional Casein-Polysaccharide Conjugates Prepared by Controlled Dry Heating
• Synthesis and Functions of a Glycopolymer Carrying Galβ1→4(GlcNac)_3 Tetrasaccharide
• Functional Analysis of Conserved Aspartate and Histidine Residues Located Around the Type 2 Copper Site of Copper-Containing Nitrite Reductase^1
• Subsite Structure and Catalytic Mechanism of a New Glycosyltrehaloseproducing Enzyme isolated from the Hyperthermophilic Archaeum, Sulfolobus solfataricus KM1
• Production of Trehalose from Starch by Novel Trehalose-Producing Enzymes from Sulfolobus solufataricus KM1
• Asymmetric Production of _L-Carnitine from trans-Crotonbetaine by Proteus mirabilis(Microbiology & Fermentation Industry)
• Mechanism of Asymmetric Production of L-Aromatic Amino Acids from the Corresponding Hydantoins by Flavobacterium sp.(Microbiology & Fermentation Industry)
• Enzymatic Production of _L-Phenylalanine from trans-Cinnamic Acid by Endomyces lindneri(Microbiology & Fermentation Industry)
• Purification and Characterization of Novel Transglutaminase from Bacillus subtilis Spores
• Asymmetric Synthesis of S-Carboxymethyl-L-cysteine by a Chemico-enzymatic Method (Microbiological & Fermentation Industry)
• Enzymatic Production of L-Serine from 2-Oxo-oxazolidine-4-carboxylic Acid(Microbiology & Fermentation Industry)
• Gluco-Oligosaccharide and Galato-Oligosaccharide Production by Rhodotorula minuta IFO879
• Mechanism of Asymmetric Production of D-Amino Acids from the Corresponding Hydantoins by Pseudomonas sp.(Microbiology & Fermentation Industry)
• Novel Low-Power Switched Current Matched Filter for DS-CDMA Wireless Communication
• Synthesis of Artificial N-Glycopolypeptides Carrying N-Acetyllactosamine and Related Compounds and Their Specific Interactions with Lectins
• Properties of Heat-induced Soluble Aggregates of Ovalbumin Preheated in the Dry State
• Novel Enzymatic Method for the Production of Xylitol from D-Arabitol by Gluconobacter oxydans(Microbiology & Fermentation Technology)
• Haliangicin, a Novel Antifungal Metabolite Produced by a Marine Myxobacterium : 2. Isolation and Structural Elucidation
• Isolation of Isoeugenitin as a Fruiting Body Inducer for Stigmatella aurantiaca from a Soil Fungus Papulaspora sp.
• Enrichment and Phylogenetic Analysis of Moderately Thermophilic Myxobacteria from Hot Springs in Japan
• Miuraenamides A and B, Novel Antimicrobial Cyclic Depsipeptides from a New Slightly Halophilic Myxobacterium : Taxonomy, Production, and Biological Properties
• Enhanced Growth of Acidocella facilis and Related Acidophilic Bacteria at High Concentrations of Aluminum
• Transaldolase/Glucose-6-phosphate Isomerase Bifunctional Enzyme and Ribulokinase as Factors to Increase Xylitol Production from D-Arabitol in Gluconobacter oxydans(Microbiology & Fermentation Technology)
• Cloning of the Xylitol Dehydrogenase Gene from Gluconobacter oxydans and Improved Production of Xylitol from D-Arabitol(Microbiology & Fermentation Technology)
• Protease Catalyzed Reaction of Polyamine Incorporation into Protein
• Adenosine Phosphorolyzing Enzymes from Microorganisms and Ribavirin Production by the Application of the Enzyme(Microbiology & Fermentation Industry)
• Mechanism of Stereospecific Production of L-Amino Acids from the Corresponding 5-Substituted Hydantoins by Bacillus brevis(Microbiology & Fermentation Industry)
• Enzymatic Production of L-Amino Acids from the Corresponding 5-Substituted Hydantoins by a Newly Isolated Bacterium, Bacillus brevis AJ-12299(Microbiology & Fermentation Industry)
• Enzymatic Production of Ribavirin from Purine Nucleosides by Brevibacterium acetylicum ATCC 954(Microbilolgy & Fermentation Industry)
• Enzumatic Production of Ribavirin from Orotidine by Erwinia carotovora AJ 2992(Pesticide Chemistry)
• Enzymatic Production of Ribavirin from Pyrimidine Nucleosides by Enterobacter aerogenes AJ 11125(Microbiology & Fermentation Industry)
• Enzymatic Production of Ribavirin(Microbiology & Fermentation Industry)
• Production of _L-Tryptophan by 5-Fluorotryptophan and Indolmycin Resistant Mutants of Bacillus subtilis K(Microbiology & Fermentation Industry)
• Effect of L-Phenylalanine and L-Methionine on the Appearance of L-Tryptophan-Producing Mutants of Bacillus subtilis K
• Deamidation of Ovalbumin during s-Ovalbumin Conversion
• Functional Properties of Deamidated Gluten Obtained by Treating with Chymotrypsin at Alkali pH(Food & Nutrition)
• Fourth 3D Structure of the Chitosan Molecule : Conformation of Chitosan in Its Salts with Medical Organic Acids Having a Phenyl Group(Analytical Chemistry)
• Dependence of the Mechanical Properties of a Pullulan Film on the Preparation Temperature(Analytical Chemistry)
• New Haliangicin Isomers, Potent Antifungal Metabolites Produced by a Marine Myxobacterium
• Haliangicin, a Novel Antifungal Metabolite Produced by a Marine Myxobacterium : 1. Fermentation and Biological Characteristics
• Effect of a Selected Amino Acid Mixture on the Recovery from Muscle Fatigue during and after Eccentric Contraction Exercise Training(Food & Nutrition Science)
• Amino Acid Supplementation Affects Hematological and Biochemical Parameters in Elite Rugby Players(Food & Nutrition Science)
• Changes in Hematological Parameters of Athletes after Receiving Daily Dose of a Mixture of 12 Amino Acids for One Month during the Middle-and Long-distance Running Training
• Purification and Properties of Thermostable β-Tyrosinase from an Obligately Symbiotic Thermophile, Symbiobacterium thermophilum
• Purification and Properties of Thermostable Tryptophanase from an Obligately Symbiotic Thermophile, Symbiobacterium thermophilum(Microbiology & Fermentation Industry)
• Interaction between Elongation Factors 1β and 1γ from Bombyx mori Silk Gland(Biochemistry & Molecular Biology)
• Cloning and Expression of Bombyx mori Silk Gland Elongation Factor 1γ in Escherichia coli(Biochemistry & Molecular Biology)
• Expression of Elongation Factor 1β'in Escherichia coli and Its Interaction with Elongation Factor 1α from Silk Gland
• A 64K Dynamic MOS-RAM Using Short-Channel, Channel-Dope Technology : A-3: MOS DEVICE AND LIST (3)
• Physiological Races and Vegetative Compatibility Groups of Fusarium oxysporum f.sp.lactucae Isolated from Crisphead Lettuce in Japan
• Race 3, a new race of Fusarium oxysporum f. sp. lactucae determined by a differential system with commercial cultivars
• Crystalline Features of Bacterial Cellulose Altered by Chemical Agents during Biosynthesis
• Saccharification of Cellulose by Acetolysis
• Breeding of Saccharomyces Yeast for Improved Growth Rate on Acetate Medium
• Expression of Stilbene Synthase Gene in Japanese Red Pine (Pinus densiflora) Seedlings
• Characterization of Novel Cysteine Proteases from Germinating Cotyledons of Soybean [Glycine max (L. ) Merrill]
• Characterization of 30-kDa Fragments Derived from β-Conglycinin Degradation Process during Germination and Seedling Growth of Soybean
• Effects of Oxygen Tension in the Gaseous Phase on Production and Physical Properties of Bacterial Cellulose Formed under Static Culture Conditions
• Purification and Properties of Purine Nucleoside Phosphorylase from Brevibacterium acetylicum ATCC 954(Microbiology & Fermentation Industry)
• Fermentative Production of L-Proline by DL-3, 4-Dehydroproline Resistant Mutants of L-Glutamate Producing Bacteria
• Enzymatic Acyl Exchange of Triglyceride in n-Hexane
• Enzymatic Synthesis of Virazole by Purine Nucleoside Phosphorylase of Enterobacter aerogenes
• Mechanism of Purine Arabinoside Synthesis by Bacterial Transarabinosylation Reaction
• Properties of Nucleoside Phosphorylase from Enterobacter aerogenes
• L-Serine Production by Temperature-sensitive Mutants of Methanol-utilizing Bacterium Pseudomonas MS31
• Microbial Synthesis of Purine 2-Amino-2-deoxyribosides
• Microbial Synthesis of Purine Arabinosides and Their Biological Activity
• L-Serine Production Improved by Analogue-resistant Mutants of a Methanol-utilizing Bacterium
• Mycophenolic Acid Production by Drug-resistant and Methionine or Glutamic-Acid Requiring Mutants of Penicillium brevicompactum
• L-Serine Production by Methanol-utilizing Bacterium Pseudomonas MS31
• Characterization of Novel Cysteine Proteases from Germinating Cotyledons of Soybean (Glycine max (L.) Merrill).

もっと見る 閉じる

スポンサーリンク