Characterization of 30-kDa Fragments Derived from β-Conglycinin Degradation Process during Germination and Seedling Growth of Soybean

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概要

• 論文の詳細を見る

• The degradation process of β-conglycinin, a vicilin-type glycosylated storage protein of soybean seeds, during germination and seedling growth was examined by concanavalin A blotting combined with polyacrylamide gel electrophoresis. We detected and analyied the structures of key intermediary fragments of 30 kDa derived from β-conglycinin degradation, they were proved to be single-domain type subunits of β-conglycinin. We show here a degradation process of β-conglycinin: β-conglycinin is subjected to limited proteolysis at exposed regions on the molecular surface, like domain junctions, generating 30-kDa singledomain fragments before non-specific proteolysis.

• 社団法人日本農芸化学会の論文
• 1997-05-23

著者

• Suzuki S

  Hiroshima Univ. Hiroshima

• Suzuki S

  Department Of Chemistry Graduate School Of Science Osaka University

• Miwa Tatsuya

  Central Research Laboratories Ajinomoto Co. Inc.

• SHIBAI Hiroshiro

  Central Research Laboratories, Ajinomoto Co., Inc.

• Asano Mitsuko

  Department Of Agricultural Chemistry The University Of Tokyo

• Suzuki Shunichi

  Central Research Laboratories Ajinomoto Co. Inc.

• Shibai Hiroshiro

  Central Research Laboratories Ajinomoto Co. Inc.

• ASANO Minao

  Food Research & Development Laboratories

• KAWAI Misako

  Food Research & Development Laboratories

• MIWA Tatsuya

  Food Research & Development Laboratories, Ajinomoto Co., Inc.

• Kawai M

  Institute Of Life Science Ajinomoto Co. Inc.

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