Transaldolase/Glucose-6-phosphate Isomerase Bifunctional Enzyme and Ribulokinase as Factors to Increase Xylitol Production from D-Arabitol in Gluconobacter oxydans(Microbiology & Fermentation Technology)

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概要

• 論文の詳細を見る

• Xylitol production from D-arabitol by the membrane and soluble fractions of Gluconobacter oxydans was investigated. Two proteins in the soluble fraction were found to have the ability to increase xylitol production. Both of these xylitol-increasing factors were purified, and on the basis of their NH_2-terminal amino acid sequences the genes encoding both of the factors were cloned. Expression of the cloned genes in Escherichia coli showed that one of the xylitol-increasing factors is the bifunctional enzyme transaldolase/glucose-6-phosphate isomerase, and the other is ribulokinase. Using membrane and soluble fractions of G. oxydans, 3.8 g/I of xylitol were produced from 10 g/1 D-arabitol after incubation for 40 h, and addition of purified recombinant transaldolase/glucose-6-phosphate isomerase or ribulokinase increased xylitol to 5.4 g/1 respectively, confirming the identity of the xylitol-increasing factors.

• 社団法人日本農芸化学会の論文
• 2003-12-23

著者

• Yokozeki Kenzo

  AminoScience Laboratories, Ajinomoto Co., Inc.

• Suzuki S

  Department Of Chemistry Graduate School Of Science Osaka University

• Suzuki S

  Aminoscience Laboratories Ajinomoto Co. Inc.

• Tonouchi N

  Ajinomoto. Co. Inc. Kawasaki Jpn

• Yokozeki K

  Ajinomoto. Co. Inc. Kawasaki Jpn

• Yokozeki Kenzo

  Aminoscience Laboratories Ajinomoto. Co. Inc.

• Yokozeki Kenzo

  Aminoscience Laboratories Ajinomoto Co. Inc.

• TONOUCHI Naoto

  AminoScience Laboratories, Ajinomoto Co., Inc.

• SUGIYAMA Masakazu

  AminoScience Laboratories, Ajinomoto Co., Inc.

• Tonouchi Naoto

  Aminoscience Laboratories Ajinomoto. Co. Inc.

• Tonouchi Naoto

  Bio-polymer Research Co. Ltd. Ksp R&d Business-park Bldg.

• Suzuki Shunichi

  Central Research Laboratories Ajinomoto Co. Inc.

• SUZUKI Shun-ichi

  Aminoscience Laboratories, Ajinomoto Co., Ltd.

• Suzuki Shun-ichi

  Aminoscience Laboratories Ajinomoto Co. Ltd.

• Sugiyama Minoru

  Aminoscience Laboratories Ajinomoto. Co. Inc.

• Sugiyama M

  Aminoscience Laboratories Ajinomoto. Co. Inc.

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