Breeding of a 5-Fluorouridine-resistant Mutant with Increased Cellulose Production from Acetobacter xylinum subsp. nonacetoxidans
スポンサーリンク
概要
- 論文の詳細を見る
UDP-glucose (UDP-G), the direct precursor of cellulose, is known to be produced from UTP and glucose-1-phosphate. In an attempt to increase UTP biosynthesis, 5-fluorouridine (5-FUR : a pyrimidine analog)-resistant mutants were obtained using Acetobacter xylinum subsp. nonacetoxidans 757 as the parent strain. One of the 5-FUR-resistant mutants, FUR-35,showed about 40% higher cellulose productivion compared to the parent strain. Intracellular levels of UTP and UDP-G in FUR-35 was found to be higher than those in the parent strain. The carbamyl phosphate synthetase II (CPS) activity of FUR-35 was higher than that of the parent strain and the feedback inhibition of CPS by UTP in FUR-35 had been released compared with that in the parent strain. These results suggest that the increased cellulose production of FUR-35 was attributable to its higher of intracellular UDPG level resulting from increased UTP biosynthesis.
- 社団法人日本農芸化学会の論文
- 1998-07-23
著者
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Tonouchi N
Ajinomoto. Co. Inc. Kawasaki Jpn
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Ishikawa A
Department Of Bioscience Fukui Prefectural University
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Ishikawa Atsushi
Bio-polymer Research Co. Ltd. :(present Address)nakano Central Institute Nakano Vinegar Co. Ltd.
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Tonouchi Naoto
Aminoscience Laboratories Ajinomoto. Co. Inc.
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Tonouchi Naoto
Bio-polymer Research Co. Ltd.
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Tonouchi Naoto
Bio-polymer Research Co. Ltd. Ksp R&d Business-park Bldg.
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YOSHINAGA FUMIHIRO
Bio-Polymer Research Co. Ltd.,
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Tsuchida Takayasu
Bio-Polymer Research Co.Ltd., KSP
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Yoshinaga F
Bio-polymer Research Co. Ltd.
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Yoshinaga Fumihiro
Bio-polymer Research Co. Ltd.
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Tsuchida T
Bio-polymer Research Co. Ltd.
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Tsuchida Takayasu
Central Research Laboratories Of Ajinomoto Co. Inc.
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Tsuchida Takayasu
Bio-polymer Research Co. Ltd.
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Tsuchida T
Bio-polymer Res. Co. Ltd.
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