Purification and Characterization of Exo-1, 4-β-Glucosidase from Acetobacter xylinum BPR2001
スポンサーリンク
概要
- 論文の詳細を見る
An exo-1, 4-β-glucosidase(EC3. 2. 1. 74 ; G3ase)was obtained from the supernatant of cultured Acetobacter xylinum subsp. sucrofermentans BPR2001 and purified to homogeneity by ammonium sulfate precipitation, cation-exchange, gel-filtration and hydrophobic interaction chromatography. The enzyme migrated to a position corresponding to 81.2kDa on SDS-polyacrylamide gel electrophoresis under both non-reducing and reducing conditions, suggesting that this enzyme is a monomer polypeptide. The isoelectric point was 6.0. N-Bromosuccinimide inhibited the activity of exo-1, 4-β-glucosidase completely, whereas sulfhydryl reagents did not. The K_m and V_<max>for the hydrolysis of cellotriose as substrate were 3.7 mM and 7.4 μmol/min/mg, respectively. The enzyme specifically cleaved the non-reducing ends of β-glucosyl linkages of cellotriose or larger cello-oligosaccharides, 4-methylumberiferryl-and p-nitrophenyl-β-D-glucosides, but cellobiose was hydrolyzed only slightly and salicin not at all. The enzyme catalyzes the hydrolysis of glucosidic linkages in such a manner that the product retains the anomeric configuration of the substrate.
- 社団法人日本生物工学会の論文
- 1998-06-25
著者
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Tahara N
Wood Research Institute Kyoto University
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Nakai Tomonori
Wood Research Inst. Kyoto Univ.
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Tonouchi N
Ajinomoto. Co. Inc. Kawasaki Jpn
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Tonouchi Naoto
Aminoscience Laboratories Ajinomoto. Co. Inc.
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Tonouchi Naoto
Bio-polymer Research Co. Ltd.
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Tonouchi Naoto
Bio-polymer Research Co. Ltd. Ksp R&d Business-park Bldg.
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YANO HISATO
Bio-Polymer Research Co. Ltd.,
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YOSHINAGA FUMIHIRO
Bio-Polymer Research Co. Ltd.,
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TAHARA NAOKI
Bio-Polymer Research Co., Ltd.
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Yano Hisato
Bio-polymer Research Co. Ltd. Ksp R&d Business-park Bldg
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Yoshinaga F
Bio-polymer Research Co. Ltd.
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Yoshinaga Fumihiro
Bio-polymer Research Co. Ltd.
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Yano Hisato
Bio-Polymer Research Co. Ltd. , KSP R&D Business-Park Bldg
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