Improvement of L-Proline Production by Sulfaguanidine Resistant Mutants Derived from L-Glutamic Acid-producing Bacteria(Microbiology & Fermentation Industry)

概要

論文の詳細を見る
Excellent L-proline producers were screened for among sulfaguanidine resistant mutants derived from three typical L-glutamic acid-producing bacteria: Brevibacterium flavum, B. lactofer-mentum, and C. glutamicum. The best strain, No. 199, is a sulfaguanidine resistant mutant derived from an isoleucine auxotroph of B. flavum 2247 by nitrosoguanidine. Strain No. 199 produced 35 mg/ml of L-proline after 72hr of cultivation with 10% glucose as a carbon source. The strain also accumulated purine bases such as adenine, guanine, and hypoxanthine, i.e., degradation products of purine nucleotides. In the mutant, 1.6〜2.0 fold more intracellular ATP was found than that in the parent strain; it is a substrate of glutamate kinase relating to L-proline biosynthesis. On the contrary, the levels of intracellular glutamic acid, a substrate of glutamate kinase, were similar among these strains. It was confirmed that the increment of internal ATP, which was important in the L-proline production mechanism, was very effective in the improvement of L-proline producers.
社団法人日本農芸化学会の論文
1986-09-23