Synthesis of Asymmetrically Labeled Sucrose by a Recombinant Sucrose Synthase
スポンサーリンク
概要
- 論文の詳細を見る
About 80% of radioactivity was recovered in asymmetrically labeled sucrose from UDP-[^<14>C]glucose or [^<14>C]fructose with recombinant mung bean sucrose synthase expressed in Escherichia coli harboring pEB-01. This high recovery is due to the fact that the enzyme conserving the activity of sucrose synthase has a similar affinity for UDP-glucose and fructose to an intact enzyme from the mung bean, but a lower affinity for sucrose.
- 社団法人日本農芸化学会の論文
- 1997-11-23
著者
-
Tahara N
Wood Research Institute Kyoto University
-
Nakai Tomonori
Wood Research Inst. Kyoto Univ.
-
Mori H
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
-
Tonouchi N
Ajinomoto. Co. Inc. Kawasaki Jpn
-
Mori Hironori
Department Of Biotechnology Faculty Of Agriculture The University Of Tokyo
-
Sakai Fukumi
Wood Research Institute, Kyoto University
-
Hayashi Takahisa
Wood Research Institute, Kyoto University
-
Sakai F
Kyoto Univ.
-
Tonouchi Naoto
Aminoscience Laboratories Ajinomoto. Co. Inc.
-
Tonouchi Naoto
Bio-polymer Research Co. Ltd.
-
Tonouchi Naoto
Bio-polymer Research Co. Ltd. Ksp R&d Business-park Bldg.
-
Tsuchida Takayasu
Bio-Polymer Research Co.Ltd., KSP
-
Mori Hitoshi
Faculty of Agriculture, Nagoya University
-
Hayashi Tomoyuki
Laboratory Of Engineered Wood And Joints Department Of Wood Engineering Forestry And Forest Products
-
Hayashi Tomoyuki
Engineered Timber And Joints Laboratory Forestry And Forest Products Research Institute
-
Hayashi T
Wood Research Institute Kyoto University
-
Sakai Fukumi
Wood Research Institute Kyoto University
-
Hayashi Takahisa
Wood Res. Inst. Kyoto Univ.
-
Hayashi T
Laboratory Of Engineered Wood And Joints Department Of Wood Engineering Forestry And Forest Products
-
Tsuchida T
Bio-polymer Research Co. Ltd.
-
Tsuchida Takayasu
Central Research Laboratories Of Ajinomoto Co. Inc.
-
Tsuchida Takayasu
Bio-polymer Research Co. Ltd.
-
Tsuchida T
Bio-polymer Res. Co. Ltd.
-
HAYASHI Takahisa
Wood Res. Inst. , Kyoto University, Gokasho, Uji, Kyoto
関連論文
- Evaluation of the Cytotoxic Activity of Chiral (E)-13-Hydroxy-10-oxo-11-octadecenoic Acid and Its Lactone
- Antitumor Activity of(10Ε, 12Ζ)-9-Hydroxy-10, 12-Octadecadienoic Acid from Rice Bran
- Cloning and Sequencing of a cDNA for Poplar Endo-1,4-β-Glucanase : GROWTH AND DEVELOPMENT : GENE STRUCTURE AND EXPRESSION
- Purification and Properties of a Wall-Bound Endo-1,4-β-Glucanase from Suspension-Cultured Poplar Cells : PROTEINS, ENZYMES AND METABOLISM
- Endo-1,4-β-glucanase in Suspension-cultured Poplar Cells
- Outdoor exposure tests of structural laminated veneer lumber (II) : evaluation of the strength properties after nine years
- Outdoor exposure tests of structural laminated veneer lumber I : evaluation of the physical properties after six years
- Purification of Xyloglucanase from Auxin-treated Pea Stems
- Coenzyme Specificity of Enzymes in the Oxidative Pentose Phosphate Pathway of Gluconobacter oxydans(Microbiology & Fermentation Technology)
- Construction of a Vector Plasmid for Use in Gluconobacter oxydans(Microbiology & Fermentation Technology)
- Lignan production in Daphne odora cell cultures
- Formation of callose from sucrose in cotton fiber microsomal membranes
- An Increase in Apparent Affinity for Sucrose of Mung Bean Sucrose Synthase Is Caused by In Vitro Phoshorylation or Directed Mutagenesis of Ser^
- Subsite Structure of Exo-1, 4-β-Glucosidase from Acetobacter xylinum BPR2001
- Purification and Characterization of Exo-1, 4-β-Glucosidase from Acetobacter xylinum BPR2001
- CALLOSE BIOSYNTHESIS FROM SUCROSE IN COTTON MEMBRANES
- Synthesis of Asymmetrically Labeled Sucrose by a Recombinant Sucrose Synthase
- A Beta-Glucosidase Gene Downstream of the Cellulose Synthase Operon in Cellulose-producing Acetobacter
- Expression and Characterization of Sucrose Synthase from Mung Bean Seedlings in Escherichia coli
- Two Types of Cellulase Activity Produced by a Cellulose-Producing Acetobacter Strain
- Genomic DNA Cloning of Poplar Endo-1, 4-β-Glucanase
- PROPERTIES OF RECOMBINANT SUCROSE SYNTHASE
- EXPRESSION OF SUCROSE SYNTHASE CDNA AND ITS ACTIVITY IN ESCHERICHIA COLI
- Addition of a Small Amount of an Endoglucanase Enhances Cellulose Production by A cetobacter xylinum
- Expression Analysis of Endo-1,4-β-Glucanase Genes, PopCel1 and PopCel2 in Poplar Cells
- Expression of Cellulases in Poplar Cells
- Breeding of a 5-Fluorouridine-resistant Mutant with Increased Cellulose Production from Acetobacter xylinum subsp. nonacetoxidans
- Acetobacter xylinum Mutant with High Cellulose Productivity and an Ordered Structure
- Relationship between Sulfaguanidine Resistance and Increased Cellulose Production in Acetobacter xylinum BPR3001E
- Increase in Cellulose Production by Sulfaguanidine-resistant Mutants Derived from Acetobacter xylinum subsp. sucrofermentans
- Cloning and Expression of the Momordica charantia Trypsin Inhibitor II Gene in Silkworm by using a Baculovirus Vector
- ISOLATION OF XYLOGLUCAN SYNTHASE
- Xyloglucan Endotransglycosylation in Suspension-cultured Poplar Cells
- Characterization of Xyloglucan Endotransglycosylation in Suspension-cultured Poplar Cells
- Characterization of the adsorption of Xyloglucan to Cellulose : STRUCTURE AND FUNCTION OF CELLS
- Effects of the Degree of Polymerization on the Binding of Xyloglucans to Cellulose : STRUCTURE AND FUNCTION OF CELLS
- FUNCTIONAL ANALYSIS OF CELLULOSE-SYNTHASE-LIKE GENES
- An Established Hybridoma Clone Producing a Monoclonal Antibody against Vibrio anguillarum
- DEGRADATION OF PLANT CELL WALLS BYVARIOUS ENDO-1, 4-β-GLUCANASES
- Shear tests of double shear plate connector joints in sugi-Japanese larch composite glulam beams
- Effect of moisture content of members on mechanical properties of timber joints
- Dynamic excitation and static loading tests of glulam lattice floor
- Ideal utilization of forest resources for sustainable society
- Degree of Polymerization of Cellulose from Acetobacter xylinum BPR2001 Decreased by Cellulase Produced by the Strain
- Expression of YAV Proteins and Vaccination against Viral Ascites among Cultured Juvenile Yellowtail
- Localization of Xyloglucan in the Macromolecular Complex Composed of Xyloglucan and Cellulose in Pea Stems : STRUCTURE AND FUNCTION OF CELLS
- CLONING OF cDNA INVOLVING TENSION WOODFORMATION
- Changes in Levels of mRNAs for Cell Wall-Related Enzymes in Growing Cotton Fiber Cells
- Reusability of Broth Supernatant as Medium
- Simple and Efficient Procedure for Removing the 34kDa Allergenic Soybean Protein, Gly m I, from Defatted Soy Milk
- Mass Production of Lipids by Lipomyces starkeyi in Microcomputer-Aided Fed-Batch Culture
- Effect of Carbon Dioxide on Growth of Microorganisms in Fed-Batch Cultures
- Transaldolase/Glucose-6-phosphate Isomerase Bifunctional Enzyme and Ribulokinase as Factors to Increase Xylitol Production from D-Arabitol in Gluconobacter oxydans(Microbiology & Fermentation Technology)
- Cloning of the Xylitol Dehydrogenase Gene from Gluconobacter oxydans and Improved Production of Xylitol from D-Arabitol(Microbiology & Fermentation Technology)
- Specific Binding of Allergenic Soybean Protein Gly m Bd 30K with α'- and α-Subunits of Conglycinin in Soy Milk
- Expression System for Foreign Genes using Protoplasts from Liriodendron tulipifera L. Suspension Culture
- BIODEGRADATION OF CELLULOSE IN SUSPENSION-CULTURED POPLAR CELLS
- Transient Expression of Goat Growth Hormone Gene in Poplar (Populus alba L.) Protoplasts : A Quick Method for Detection of Foreign Gene Expression in mRNA Level
- Increased Cellulose Production from Sucrose with Reduced Levan Accumulation by an Acetobacter Strain Harboring a Recombinant Plasmid
- The Characterization of Acetic Acid Bacteria Efficiently Producing Bacterial Cellulose from Sucrose : The Proposal of Acetobacter xylinum subsp. nonacetoxidans subsp. Nov.
- High Rate Production in Static Culture of Bacterial Cellulose from Sucrose by a Newly Isolated Acetobacter Strain
- Screening of Bacterial Cellulose-producing Acetobacter Strains Suitable for Sucrose as a Carbon Source
- Construction of a Novel Hydroxyproline-Producing Recombinant Escherichia coli by Introducing a Proline 4-Hydroxylase Gene
- Reduction of HIV-1 Infectivity and Reverse Transcriptase Activity by High Hydrostatic Pressure Treatment
- Enzymatic Production of trans-4-Hydroxy-L-proline by Regio- and Stereospecific Hydroxylation of L-Proline
- Expression of Bombyx Family Fungal Protease Inhibitor F from Bombyx mori by Baculovirus Vector
- Specific Proline and Hydroxyproline Detection Method by Post-column Derivatization for High-performance Liquid Chromatography
- Cloning of a New cryIA(a) Gene from Bacillus thuringiensis Strain FU-2-7 and Analysis of Chimaeric CryIA(a) Proteins for Toxicity
- Screening of Bacterial Cellulose-producing Acetobacter Strains Suitable for Agitated Culture
- Increased Cellulose Production from Sucrose by Acetobacter after Introducing the Sucrose Phosphorylase Gene
- Characterization of the Biosynthetic Pathway of Cellulose from Glucose and Fructose in Acetobacter xylinum
- ARRANGEMENT OF CORTICAL MICROTUBULES AND β-GLUCAN SYNTHASES IN PLASMA MEMBRANE GHOSTS OF TOBACCO BY-2 CELLS
- An O-Methyltransferase (OMT) cDNA Clone in Japanese Red Pine (Pinus densiflora) Seedlings
- A Host-Vector System for a Cellulose-Producing Acetobacter Strain
- Changes in Levels of mRNAs for Cell Wall-related Enzymes in Growing Cotton Cells
- GENE EXPRESSION OF WALL-MODIFYING ENZYMES IN THE GROWING COTTON FIBER CELLS
- Transferase activity of GhCesA2 (putative cotton cellulose 4-β-glucosyltransferase) expressed in Pichia pastoris
- A Homologue of EGL1 Encoding Endo-1,4-β-glucanase in Elongating Pea Stems
- Involvement of Xyloglucan Endotransglycosylase in Cellulose Biosynthesis
- OVEREXPRESSION OF POPLAR ENDO-1,4-β-GLUCANASE IN ARABIDOPSIS
- Cloning of Cotton Homologs of bcsA Gene Encoding Cellulose β-1,4-Glucosyltransferase
- LOCALIZATION OF pcsA POLYPEPTIDE IN COTTON CELLS
- Cloning and Sequencing of Cotton Homologs of bcsA Gene Encoding Cellulose 4-β-Glucosyltransferase
- Expression of Gene Encoding Endo-1, 4-β-glucanase in Suspension-cultured Poplar (Poplus alba L.) Cells
- Prosthetic Group of Aldehyde Dehydrogenase in Acetic Acid Bacteria Not Pyrroloquinoline Quinone
- Shear tests of timber joints composed of sugi composite glulam beams using newly developed steel connectors
- Compositional Analysis of the Oligosaccharide Units of Xyloglucans from Suspension-cultured Poplar Cells
- Research Progress in Production of Bacterial Cellulose by Aeration and Agitation Culture and Its Application as a New Industrial Material
- Simultaneous expression of stilbene synthase genes in Japanese red pine (Pinus densiflora) seedlings
- Characterization of Stilbene Synthase Genes in Japanese Red Pine (Pinus densiflora)
- GENE EXPRESSION OF ENDO-1, 4-β-GLUCANASE, ENDOXYLOGLUCAN TRANSFERASE AND EXPANSIN IN PEA STEMS
- EXPRESSION OFENDO-1, 4-β-GLUCANASE INSUSPENSION CULTURED POPLAR CELLS
- Production of Endo-1,4-β-glucanase Activity: A Simple Method for Suspension Culture of Poplar Cells
- Purification and Properties of an Extracellular Endo-1,4-β-Glucanase from Suspension-Cultured Poplar Cells
- Macromolecular Complexes of Xyloglucan and Cellulose Obtained by Annealing : STRUCTURE AND FUNCTION OF CELLS
- Carbohydrate Analysis of an Interspecific Hybrid between Onion and Garlic
- XYLOGLUCAN STORY IN THE APOPLAST
- A PECULIAR CRYSTALLINE STRUCTURE IN NEURONS OF RABBITS TREATED WITH VINCRISTINE
- High density cultivation of biomass in fed-batch system with DO-stat.
- HISTOLOGICAL STUDIES ON THE INTRANUCLEAR INCLUSIONS OF THE MELANIN-PIGMENTED NERVE CELLS IN THE SUBSTANTIA NIGRA AND THE NUCLEUS LOCI CAERULEI