Specific Proline and Hydroxyproline Detection Method by Post-column Derivatization for High-performance Liquid Chromatography
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概要
- 論文の詳細を見る
A specific detection method for hydroxyproline by post-column derivatization with 7-chloro-4-nitrobenzo-2-oxa-1, 3-diazole was developed after chiral resolution by ligand-exchange chromatography. The addition of ethylenediaminetetraacetic acid to the reaction mixture was effective to achieve the post-column reaction. The calibration curve for trans-4-hydroxy-L-proline shows linearity from 7. 6pmol up to 7600pmol.
- 社団法人日本農芸化学会の論文
- 1995-09-23
著者
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Mori H
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Mori Hironori
Department Of Biotechnology Faculty Of Agriculture The University Of Tokyo
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Ozaki A
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Ozaki Akio
Technical Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Ozaki Akio
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.:(present Office)technical Research Laboratori
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MORI HIDEO
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co.Ltd.
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SHIBASAKI Takeshi
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
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Shibasaki Takeshi
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.: (present Address) Technical Research Laborat
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