Enzymatic Production of D-Glutamate from L-Glutamate by a Glutamate Racemase

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概要

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• D-Glutamate was produced from L-glutamate by two successive cellular reactions with a glutamate racemase produced by Escherichia coli TM93 harboring a plasmid containing a glutamate racemase gene from Lactobacillus brevis ATCC 8287 and a glutamate decarboxylase produced by E. coli ATCC 11246. L-Glutamate was first racemized to DL-glutamate at pH 8.5 and L-glutamate was then decarboxylated at pH 4.2. Starting from 100 g/l of L-glutamate, 50 g/l of D-glutamate remained after 15 h reaction.

• 社団法人日本生物工学会の論文
• 1995-01-25

著者

• Yagasaki Makoto

  Technical Research Laboratories, Kyowa Hakko Bio Co., Ltd.

• Azuma M

  Osaka City Univ. Osaka Jpn

• Yagasaki M

  Kyowa Hakko Kogyo Co. Ltd. Yamaguchi Jpn

• Yagasaki Makoto

  Technical Research Laboratories Kyowa Hakko Bio Co. Ltd.

• Yagasaki Makoto

  Technical Research Laboratories Kyowa Hakko Kogyo Co. Ltd.

• Azuma Masaki

  Technical Research Laboratories Kyowa Hakko Kogyo Co. Ltd.

• Ozaki A

  Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.

• Ozaki Akio

  Technical Research Laboratories Kyowa Hakko Kogyo Co. Ltd.

• Ishino Shuichi

  Technical Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.

• AKIO OZAKI

  Technical Research Laboratories, Kyowa Hakko Kogyo Co. Ltd.,

• Ishino S

  Kyowa Hakko Kogyo Co. Ltd. Yamaguchi Jpn

• Ishino Shuichi

  Technical Research Laboratories Kyowa Hakko Kogyo Co. Ltd.

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