Cloning and Sequencing of the meso-Diaminopimelate-D-dehydrogenase (ddh) Gene of Corynebacterium glutamicum(Microbiology & Fermentation Industry)
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概要
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The ddh gene of Corynebacterium glutamicum encoding meso-diaminopimelate (meso-DAP)-D-dehydrogenase (DDH) involved in the lysine biosynthesis was cloned in a DAP auxotroph (dapD4) of Escherichia coli by complementation of the DAP auxotroph. Deletion analysis revealed that a 〜1.7-kb XhoI-KpnI fragment contained the ddh structure gene. The specific activity of DDH was increased fourteen-fold when C. glutamicum was transformed with a recombinant plasmid harbouring the cloned ddh gene. Furthermore, the ddh gene has been sequenced [S. Ishino et al., Nucleic Acids Res., 15, 3917 (1987)] and some properties of the ddh gene are discussed.
- 社団法人日本農芸化学会の論文
- 1988-11-23
著者
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Araki Kazumi
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Araki K
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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YAMAGUCHI Kazuo
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
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Ishino S
Kyowa Hakko Kogyo Co. Ltd. Yamaguchi Jpn
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Mizukami Toru
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Katsumata Ryoichi
Tokyo Researach Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Katsumata R
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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ISHINO Shuichi
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
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Yamaguchi K
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Yamaguchi Kazuo
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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