Magnesium Phosphates Stimulate Microbial Conversion of Glycine to L-Serine by Release from Regulation by Ammonium Ions : Microbial Conversion of Glycine to L-Serine with Nocardia butanica (V).

スポンサーリンク

概要

• 論文の詳細を見る

• We have reported the stimulation of conversion of glycine to L-serine with Nocardia butanica mutants by tribasic magnesium phosphate (t-MgP). The stimulation was further studied with respect to the effect of t-MgP on the regulation of L-serine formation by ammonium ions and glucose with resting cells of a wild-type strain grown in a chemically defined medium supplemented with t-MgP or calcium carbonate. High concentrations of ammonium ions and/or glucose interfered with L-serine formation in a resting cell system of CaCO_3-grown cells. Amines, other sugars, and organic acids of the tricarboxylic acid cycle were also interfering. When the system was supplemented with a mixture of monobasic MgP (m-MgP) and t-MgP, L-serine formation became free from the interference by ammonium ions, while the interference by glucose persisted. Similarly, L-serine formation in a resting cell system of t-MgP-grown cells was free from the interference by ammonium ions, but was subject to the interference by glucose. The rate of L-serine formation was higher in the t-MgP-grown cell system than in the CaCO_3-grown cell system. Addition of m-MgP to the resting cell systems suppressed L-serine degradation. The effect was applied to further improvement of L-serine production in growing cultures of a mutant strain, KY-7990,yielding 19 mg/ml of L-serine. Thus it is concluded that the addition of t-MgP resulted in the release of L-serine formation with N. butanica from the regulation by ammonium ions, and in the maintenance of pH values in a favorable range, which led to the enhancement of L-serine production from glycine.

• 公益社団法人日本生物工学会の論文
• 1981-12-25

著者

• OMURA Satoshi

  School of Pharmaceutical Sciences, Kitasato University, Research Center for Biological Function, The

• Tanaka H

  The Kitasato Institute

• Tanaka Y

  School Of Pharmaceutical Sciences Kitasato University The Kitasato Institute

• Takada Y

  School Of Pharmaceutical Sciences Kitasato University And Research Center For Biological Function Th

• Araki Kazumi

  Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.

• Tanaka Y

  Research Center For Biological Function

• Nakayama Kiyoshi

  Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.

• Tanaka Haruo

  School Of Pharmaceutical Sciences Kitasato University

• TANAKA YOSHITAKE

  School of Pharmaceutical Sciences, Kitasato University and The Kitasato Institute

• Tanaka Yoshitake

  School Of Pharmaceutical Sciences Kitasato University And The Kitasato Institute

• Omura Satoshi

  School Of Pharmaceutical Sciences Kitasato University And Kitasato Institute

• Tanaka Yoshitake

  School Of Pharmaceutical Sciences Kitasato University And Research Center For Biological Function The Kitasato Institute

関連論文

• 10.酢酸菌のグルコン酸酸化にかかわる第3の膜酵素・FADグルコン酸脱水素酵素(第419回研究協議会研究発表要旨,ビタミンB研究委員会)
• 4.キノプロテインおよびキノヘモプロテイン・アルコール脱水素酵素の構造と機能(B群ビタミン酵素構造機能解析の新展開,シンポジウム(平成19年度),ビタミンB研究委員会)
• 11. 新規なキノプロテイン・キナ酸脱水素酵素とシキミ酸生産系の開発(第407回研究協議会研究発表要旨,ビタミンB研究委員会)
• Molecular Target of Piperine in the Inhibition of Lipid Droplet Accumulation in Macrophages(Biochemistry)
• Generation of reactive oxygen species from conventional laboratory media(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Total Synthesis of Malformin C, an Inhibitor of Bleomycin-Induced G2 Arrest
• Fungal Malformins Inhibit Bleomycin-Induced G2 Checkpoint in Jurkat Cells(Highlighted paper selected by Editor-in-chief,Biochemistry)
• 1S-Ep05 耐熱性酢酸菌を使った酸化発酵による有用物質生産系の開発(伝統的醗酵微生物の新しい利用展開,シンポジウム,伝統の技と先端科学技術の融合)
• Production of the New Antibiotic Tetrahydrokalafungin by Transformants of the Kalafungin Producer Streptomyces tanashiensis
• Isolation of Restriction-reduced Mutants from Streptomyces(Microbiology & Fermentation Industry)
• Biochemical and Spectroscopic Properties of Cyanide-Insensitive Quinol Oxidase from Gluconobacter oxydans
• Guadinomines, Type III Secretion System Inhibitors, Produced by Streptomyces sp. K01-0509 : II. Physico-chemical Properties and Structure Elucidation
• Guadinomines, Type III Secretion System Inhibitors, Produced by Streptomyces sp. K01-0509 : I. Taxonomy, Fermentation, Isolation and Biological Properties
• 琉球大学農学部「亜熱帯生物資源科学科,発酵・生命科学コース」と,設置予定の「泡盛学寄附講座」の紹介(九州支部,Branch Spirit)
• 2P030 酢酸菌Gluconobacter frateurii由来L-sorbose reductaseのX線結晶構造解析(蛋白質-構造機能相関,第48回日本生物物理学会年会)
• Lariatins, Novel Anti-mycobacterial Peptides with a Lasso Structure, Produced by Rhodococcus jostii K01-B0171
• Application of PCR for Selection of Gram-Positive Bacteria with High DNA G+C Content among New Isolates
• Citrinamides, New Potentiators of Antifungal Miconazole Activity, Produced by Penicillium sp. FKI-1938
• Tensyuic Acids, New Antibiotics Produced by Aspergillus niger FKI-2342
• Fungal Citridone D Having a Novel Phenylfuropyridine Skeleton
• Phenatic Acids A and B, New Potentiators of Antifungal Miconazole Activity Produced by Streptomyces sp. K03-0132
• Takanawaenes, Novel Antifungal Antibiotics Produced by Streptomyces sp. K99-5278 I. Taxonomy, Fermentation, Isolation and Biological Properties
• The Neuritogenesis Inducer Lactacystin Arrests Cell Cycle at Both Go/G1 and G2 Phases in Neuro 2a Cells
• Direct Production of 5-Oxo Derivatives of Avermectins by a Recombinant Strain of Streptomyces avermitilis
• Arohynapene D, a New Anticoccidial Agent Produced by Penicillium sp.FO-2295
• Fudecalone, a New Anticoccidial Agent Produced by Penicillium sp.FO-2030
• Terpendoles, Novel ACAT Inhibitors Produced by Albophoma yamanashiensis II.Structure Elucidation of Terpendoles A, B, C and D
• Terpendoles, Novel ACAT Inhibitors Produced by Albophoma yamanashiensis I.Production, Isolation and Biological Properties
• Isolation and Characterization of Covalently Closed Circular DNA Associated with Chromosomal and Membrane Fraction from Streptomyces ambofaciens
• Genetic and Biochemical Features of Spiramycin Biosynthesis in Streptomyces ambofaciens--Curing,Protoplast Regeneration and Plasmid Transfer
• Extraction and Characterization of Plasmids from Macrolide Antibiotic-Producing Streptomycetes
• Biosynthesis of Lactacystin
• In Vitro and in Vivo Antimalarial Activities of a Non-glycosidic 18-Membered Macrolide Antibiotic, Borrelidin, against Drug-resistant Strains of Plasmodia
• Anti-atherosclerotic Activity of Triacsin C, an Acyl-CoA Synthetase Inhibitor
• Verticipyrone, a New NADH-fumarate Reductase Inhibitor, Produced by Verticillium sp. FKI-1083
• The Selectivity of Beauveriolide Derivatives in Inhibition toward the Two Isozymes of Acyl-CoA: cholesterol Acyltransferase
• Selective Inhibition of Acyl-CoA : cholesterol Acyltransferase 2 Isozyme by Flavasperone and Sterigmatocystin from Aspergillus Species
• Selectivity of Pyripyropene Derivatives in Inhibition toward Acyl-CoA : cholesterol Acyltransferase 2 Isozyme
• Cyslabdan, a New Potentiator of Imipenem Activity against Methicillin-resistant Staphylococcus aureus, Produced by Streptomyces sp. K04-0144 : II. Biological Activities
• Cyslabdan, a New Potentiator of Imipenem Activity against Methicillin-resistant Staphylococcus aureus, Produced by Streptomyces sp. K04-0144 : I. Taxonomy, Fermentation, Isolation and Structural Elucidation
• Yaequinolones, New Insecticidal Antibiotics Produced by Penicillium sp. FKI-2140 : I. Taxonomy, Fermentation, Isolation and Biological Activity
• Inhibition of Lipid Droplet Accumulation in Mouse Macrophages by Stemphone Derivatives
• Structure-activity Relationships of Stemphones, Potentiators of Imipenem Activity against Methicillin-resistant Staphylococcus aureus
• Yaequinolones, New Insecticidal Antibiotics Produced by Penicillium sp. FKI-2140 : II. Structural Elucidation
• Selectivity of Microbial Acyl-CoA : cholesterol Acyltransferase Inhibitors toward Isozymes
• Production of a New Methylated 6, 8a-Seco-6, 8a-deoxy Derivative of the Avermectins by a Transformant Strain of Streptomyces avermitilis
• Production of 6, 8a-Seco-6, 8a-deoxy Derivatives of Avermectins by a Mutant Strain of Streptomyces avermitilis
• Formaldehyde Elimination with Formaldehyde and Formate Oxidase in Membrane of Acetic Acid Bacteria(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Isochromophilones I and II, Novel Inhibitors against gp120-CD4 Binding Produced by Penicillium multicolor FO-2338 II.Structure Elucidation
• Isochromophilones I and II, Novel Inhibitors against gp120-CD4 Binding Produced by Penicillium multicolor FO-2338 I.Screening, Taxonomy, Fermentation, Isolation and Biological Activity
• Three-Dimensional Structure-Activity Relationship Analysis between Motilin and Motilide Using Conformational Analysis and a Novel Molecular Superposing Method
• Enzymatic Preparation of Metabolic Intermediates, 3-Dehydroquinate and 3-Dehydroshikimate, in the Shikimate Pathway
• Purification and Properties of NADP-Dependent Shikimate Dehydrogenase from Gluconobacter oxydans IFO 3244 and Its Application to Enzymatic Shikimate Production
• High Shikimate Production from Quinate with Two Enzymatic Systems of Acetic Acid Bacteria
• Conversion of Capsular Polysaccharide, Involved in Pellicle Formation, to Extracellular Polysaccharide by galE Deletion in Acetobacter tropicalis
• Control of Avermectin Biosynthesis in Streptomyces avermitilis for the Selective Production of a Useful Component
• Physiological Regulation of Sporulation of Kitasatosporia setae in Submerged Culture
• Amino Acid Production from Methanol by Methylobacillus glycogenes Mutants : Isolation of L-Glutamic Acid Hyper-producing Mutants from M. glycogenes Strains, and Derivation of L-Threonine and L-Lysine-producing Mutants from Them
• A Tightly Bound Quinone Functions in the Ubiquinone Reaction Sites of Quinoprotein Alcohol Dehydrogenase of an Acetic Acid Bacterium, Gluconobacter suboxydans
• A Novel 3-Dehydroquinate Dehydratase Catalyzing Extracellular Formation of 3-Dehydroshikimate by Oxidative Fermentation of Gluconobacter oxydans IFO 3244
• Energy Metabolism of a Unique Acetic Acid Bacterium, Asaia bogorensis, That Lacks Ethanol Oxidation Activity
• Distinct Physiological Roles of Two Membrane-Bound Dehydrogenases Responsible for D-Sorbitol Oxidation in Gluconobacter frateurii
• Preparation of Enzymes Required for Enzymatic Quantification of 5-Keto-D-gluconate and 2-Keto-D-gluconate
• 耐熱性酢酸菌を使った酸化発酵による有用物質生産系の開発(伝統的発酵微生物の新しい利用展開)
• Cloning and Sequencing of the meso-Diaminopimelate-D-dehydrogenase (ddh) Gene of Corynebacterium glutamicum(Microbiology & Fermentation Industry)
• 6.ピロロキノリンキノン(PQQ)高生産菌Methylobacillus glycogenesの再分類とPQQ生産条件の検討(第424回研究協議会研究発表要旨,ビタミンB研究委員会)
• Selective, High Conversion of D-Glucose to 5-Keto-D-gluoconate by Gluconobacter suboxydans
• ^C NMR Studies of Lysine Fermentation with a Corynebacterium glutamicum Mutant(Microbiology & Fermentation Industry)
• Magnesium Phosphates Stimulate Microbial Conversion of Glycine to L-Serine by Release from Regulation by Ammonium Ions : Microbial Conversion of Glycine to L-Serine with Nocardia butanica (V).
• Cultural Conditions for Microbial Conversion of Glycine into L-Serine in the Presence of Tribasic Magnesium Phosphate : Microbial Conversion of Glycine into L-Serine with Nocardia butanica (III)
• Stimulation of Microbial Conversion of Glycine into L-Serine by Magnesium Phosphate : Microbial Conversion of Glycine into L-Serin with Nocardia butanica(II)
• Strain Improvement of Nocardia butanica for Microbial Conversion of Glycine into _L-Serine : Microbial Conversion of Glycine into _L-Serine with Nocardia butanica (I)
• Production of Lactase by Mutants of Kluyveromyces lactis(Microbiology & Fermentation Industry)
• Global Analysis of the Genes Involved in the Thermotolerance Mechanism of Thermotolerant Acetobacter tropicalis SKU1100
• Makomotindoline from Makomotake, Zizania latifolia infected with Ustilago esculenta
• 沖縄の伝統発酵食品と微生物 : 泡盛を中心に(伝統発酵食品研究の新展開〜微生物共生から探る〜)
• Generation of superoxide anions by a glycation reaction in conventional laboratory media
• 沖縄とバイオ、泡盛を飲みながら
• New verticilides, inhibitors of acyl-CoA : cholesterol acyltransferase, produced by Verticillium sp. FKI-2679
• New isochaetochromin, an inhibitor of triacylglycerol synthesis in mammalian cells, produced by Penicillium sp. FKI-4942 : II. structure elucidation
• New isochaetochromin, an inhibitor of triacylglycerol synthesis in mammalian cells, produced by Penicillium sp. FKI-4942 : I. Taxonomy, fermentation, isolation and biological properties
• The high mannose-type glycan binding lectin actinohivin : dimerization greatly improves anti-HIV activity
• Production of monapinones by fermentation of the dinapinone-producing fungus Penicillium pinophilum FKI-3864 in a seawater-containing medium
• Dinapinones, novel inhibitors of triacylglycerol synthesis in mammalian cells, produced by Penicillium pinophilum FKI-3864
• Spoxazomicins A-C, novle antitrypanosomal alkaloids produced by an endophytic actinomycete, Streptosporangium oxazolinicum K07-0460^T
• Pentacecilides, new inhibitors of lipid droplet formation in mouse macrophages, produced by Penicillium cecidicola FKI-3765-1 : I. Taxonomy, fermentation, isolation and biological properties
• Expression of two human acyl-CoA : diacylglycerol acyltransferase isozymes in yeast and selectivity of microbial inhibitors toward the isozymes
• Total synthesis of amidepsine B and revision of its absolute configuration
• 最新ビタミン学講話 : ビタミン発見100周年を記念して(18)PQQ(ピロロキノリンキノン)
• Nosokomycins, new antibiotics discovered in an in vivo-mimic infection model using silkworm larvae. II : Structure elucidation
• Nosokomycins, new antibiotics discovered in an in vivo-mimic infection model using silkworm larvae I : Fermentation, isolation and biological properties
• Absolute stereochemistry of pentacecilides, new inhibitors of lipid droplet formation in mouse macrophages, produced by Penicillium cecidicola FKI-3765-1
• Actinohivin : specific amino acid residues essential for anti-HIV activity
• Xanthoradone C, a new potentiator of imipenem activity against methicillin-resistant Staphylococcus aureus, produced by Penicillium radicum FKI-3765-2
• Calpinactam, a new anti-mycobacterial agent, produced by Mortierella alpina FKI-4905
• L-Arginine Production by Arginine Analog-resistant Mutants of Microorganisms
• Involvement of meso-α, ε-Diaminopimelate D-Dehydrogenase in Lysine Biosynthesis in Corynebacterium glutamicum
• Derepression of Glycine Decarboxylase Synthesis by MagnesiumPhosphate in Nocardia butanica
• Ammonia Trapping and Stimulation of L-Serine Production from Glycine by Magnesium Phosphate
• 13C NMR Studies of Histidine Fermentation with a Corynebacterium glutamicum Mutant

もっと見る 閉じる

スポンサーリンク