Identification and Cloning of the Gene Involved in the Final Step of Chlortetracycline Biosynthesis in Streptomyces aureofaciens

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概要

• 論文の詳細を見る

• For chlortetracycline biosynthesis in Streptomyces aureofaciens, the final reduction step is essential to give an antibiotic activity to its intermediate, which is catalyzed by tetracycline dehydrogenase with 7,8-dedimethyl-8-hydroxy-5-deazariboflavin (FO) as a cofactor. We identified and cloned the gene, which is essential for the biosynthesis of 6-demethyltetracycline and participates in the final step of its biosynthesis, from the genomic DNA of the 6-demethyltetracycline producer S. aureofaciens HP77. DNA sequence analysis revealed that the gene (tchA) had an open reading frame of 455 amino acids with an estimated molecular mass of 48.1 kDa. Southern hybridization analysis revealed that the tchA gene was located external to the chlortetracycline biosynthetic gene cluster in the genome. A conserved domain search of protein sequence databases indicated that TchA showed a similarity to FbiB, which is involved in the modification of FO in Mycobacterium bovis.

• 社団法人 日本農芸化学会の論文
• 2004-06-23

著者

• Nakano Tomoki

  Department Of Biochemical Science And Technology Faculty Of Agriculture Kagoshima University

• Nakano T

  Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.

• Nakano Tetsuo

  Tokyo Denki University

• DAIRI Tohru

  Biotechnology Research Center, Faculty of Engineering, Toyama Prefectural University

• Endo Hirofumi

  Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.

• Mizukami T

  Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.

• Mizukami Toru

  Tokyo Research Laboratories of Kyowa Hakko Kogyo Co., Ltd.

• Mizukami Toru

  Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.

• Katsumata R

  Graduate School Of Agricultural Science Tohoku University

• Dairi T

  Biotechnology Res. Center Toyama Prefectural Univ.

• Dairi Tohru

  Biotechnology Research Center Toyama Prefectrual University

• MIYAKE Koichiro

  Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.

• KATSUMATA Ryoichi

  Graduate school of Agricultural Science, Tohoku University

• Miyake Koichiro

  Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.

• Nakano Toshiki

  Marine Biochemistry Laboratory Gradate School Of Agricultural Science Tohoku University

• Dairi Tohru

  Hokkaido Univ. Hokkaido Jpn

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