Cloning and Nucleotide Sequence of the Gene Responsible for Chlorination of Tetracycline

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概要

• 論文の詳細を見る

• Two cosmid clones containing distinct types of self-defense gene of a 6-demethylchlortetracycline producer, Streptomyces aureofaciens NRRL3203, were isolated. The gene responsible for chlorination of tetracycline (chl gene) was subcloned from one of the cosmid clones by complementation of a chlorination-deficient mutant, using a gene cloning system for strain NRRL3203 developed in this study. The nucleotide sequence analysis of a 4.4-kb SacI-BamHI fragment containing the chl gene showed that the predicted product of the chl gene is a polypeptide of 452 amino acids, and that the chl gene was preceded by two open reading frames, which could endode polypeptides of 50 kDa and 32 kDa, respectively. A search for sequences homologous to these ORFs found that the former product strongly resembles that of the 6-hydroxylation enzyme for oxytetracycline biosynthesis, and that the latter product has a weak but significant similarity to the hydroxyindole O-methyltransferase of bovine pineal gland. By Northern blot analysis, these three genes were suggested to be polycistronically transcribed.

• 社団法人日本農芸化学会の論文
• 1995-06-23

著者

• Nakano Tomoki

  Department Of Biochemical Science And Technology Faculty Of Agriculture Kagoshima University

• Nakano T

  Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.

• Nakano Tetsuo

  Tokyo Denki University

• AISAKA KAZUO

  Tokyo Research Laboratories, Kyowa Hakko Kogyo Co.Ltd.

• Aisaka K

  Biofrontier Laboratories Kyowa Hakko Kogyo Co. Ltd.

• Aisaka K

  Japan Tobacco Inc. Osaka Jpn

• Aisaka Kazuo

  Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.

• KATSUMATA RYOICHI

  Tokyo Research Laboratories, Kyowa Hakko Kogyo Co.Ltd.

• HASEGAWA Mamoru

  Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.

• Hasegawa Mamoru

  Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.

• Dairi Tohru

  Tokyo Research Laboratories of Kyowa Hakko Kogyo Co., Ltd.

• Katsumata R

  Graduate School Of Agricultural Science Tohoku University

• Katsumata Ryoichi

  Tokyo Researach Laboratories Kyowa Hakko Kogyo Co. Ltd.

• Dairi T

  Biotechnology Res. Center Toyama Prefectural Univ.

• Nakano Toshiki

  Marine Biochemistry Laboratory Gradate School Of Agricultural Science Tohoku University

• Dairi Tohru

  Hokkaido Univ. Hokkaido Jpn

• Hasegawa Morifumi

  Laboratory of Agricultural Chemicals, Faculty of Agriculture, Ibaraki University

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