Enzymatic Production of Amoxicillin by β-Lactamase-deficient Mutants of Pseudomonas melanogenum KY 3987
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概要
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A search was undertaken for microorganisms that can catalyze the enzymatic production of amoxicillin. Pseudomonas melanogenum KY 3987 was selected from our stock cultures, β-Lactamase-deficient mutants were isolated from amoxicillin-sensitive mutants derived from this strain. These mutants became sensitive to other penicillins and cephalosporins as well as amoxicillin and did not produce β-lactamase even in the presence of an inducer, such as penicillin V. Amoxicillin was effectively synthesized from D-2-p-hydroxyphenylglycine methyl ester and 6-aminopenicillanic acid (6-APA) with these mutants as the enzyme sources. The conversion ratio from 6-APA to amoxicillin was about 90% as a mol ratio.
- 社団法人 日本農芸化学会の論文
著者
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Kawamori Mikio
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Katsumata Ryoichi
Tokyo Researach Laboratories Kyowa Hakko Kogyo Co. Ltd.
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HASHIMOTO Yukio
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
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OKACHI Ryo
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
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TAKAYAMA Kenichiro
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
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