Fermentative Production of Tryptophan by a Stable Recombinant Strain of Corynebacterium glutamicum with a Modified Serine-biosynthetic Pathway
スポンサーリンク
概要
- 論文の詳細を見る
Introduction of plasmid pKW99, which coexpresses the deregulated 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase and tryptophan-biosynthetic enzymes, into tryptophan-producing Corynebacterium glutamicum KYl0894 resulted in a marked increase (54%) in yield of tryptophan production (43g/liter), but incurred two problems. One was a decline in sugar consumption at the late stage of fermentation, and the other the loss of the plasmid in the absence of selective pressure. The retarded sugar assimilation was found to be attributed to the death of cells that arose from the detrimental action of indole, the last intermediate in the tryptophan pathway, accumulated as a by-product. A chain of these events simultaneously disappeared when serine, the other substrate of the final reaction by tryptophan synthase, was added. These results indicated that a limiting supply of serine was the cause of the decline in the sugar consumption. Thus, to increase carbon flux into serine, the gene for 3-phosphoglycerate dehydrogenase (PGD), the first enzyme in the serine pathway, was cloned from wild-type C. glutamicum ATCC 31833 and joined onto pKW99 to generate pKW9901. Strain KY10894 transformed with pKW9901 favorably consumed sugar through fermentation with accumulating little indole. Furthermore, on the basis of the observation that serine in the medium was consumed rapidly by the recombinant cells, we developed a unique plasmid stabilization system composed of KY9218 (a PGD-deficient serine-requiring strain of KY10894) and pKW9901 : In its combination, cells lacking the plasmid should not proliferate in the fermentation medium which does not contain serine. Even if selective pressure was not applied, the modified strain KY9218 with pKW9901 stably maintained the plasmid during fermentation and produced 50g/liter of tryptophan in a 61% increased yield relative to strain KY10894.
- 社団法人日本農芸化学会の論文
- 1994-04-23
著者
-
IKEDA Masato
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
-
Kino K
Kyowa Hakko Kogyo Co. Ltd. Yamaguchi Jpn
-
Ikeda Masato
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
-
KATSUMATA RYOICHI
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co.Ltd.
-
Katsumata Ryoichi
Tokyo Researach Laboratories Kyowa Hakko Kogyo Co. Ltd.
-
Nakanishi Keiko
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
-
Kino Kuniki
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
-
Kino Kuniki
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
-
Nakanishi Keiko
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
関連論文
- Transcriptome Analysis of Acetate Metabolism in Corynebacterium glutamicum Using a Newly Developed Metabolic Array(Microbiology & Fermentation Technology)
- _L-Alanine Fermentation by an Alanine Racemase-Deficient Mutant of the _-Alanine Hyperproducing Bacterium Arthrobacter oxydans HAP-1
- Transport of Aromatic Amino Acids and Its Influence on Overproducton of the Amino Acids in Corynebacterium glutamicum
- Tryptophan Production by Transport Mutants of Corynebacterium glutamicum
- A Self-defense Gene Homologous to Tetracycline Effluxing Gene Essential for Antibiotic Production in Streptomyces aureofaciens
- Conserved Organization of Genes for Biosynthesis of Chlortetracycline in Streptomyces Strains
- Cloning and Nucleotide Sequence of the Gene Responsible for Chlorination of Tetracycline
- Cloning of the ATP Phosphoribosyl Transferase Gene of Corynebacterium glutamicum and Application of the Gene to L-Histidine Production
- Fermentative Production of Tryptophan by a Stable Recombinant Strain of Corynebacterium glutamicum with a Modified Serine-biosynthetic Pathway
- Amino Acid Production from Methanol by Methylobacillus glycogenes Mutants : Isolation of L-Glutamic Acid Hyper-producing Mutants from M. glycogenes Strains, and Derivation of L-Threonine and L-Lysine-producing Mutants from Them
- Cloning and Sequencing of the meso-Diaminopimelate-D-dehydrogenase (ddh) Gene of Corynebacterium glutamicum(Microbiology & Fermentation Industry)
- Cloning of the Genes Concerned in Phenylalanine Biosynthesis in Corynebacterium glutamicum and its Application to Breeding of a Phenylalanine Producing Strain
- Enzymatic Production of Amoxicillin by β-Lactamase-deficient Mutants of Pseudomonas melanogenum KY 3987