A Self-defense Gene Homologous to Tetracycline Effluxing Gene Essential for Antibiotic Production in Streptomyces aureofaciens

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概要

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• By Northern blot analyses with DNA probes carrying 6-demethylchlortetracycline (6-DCT) biosynthetic genes from Streptomyces aureofaciens NRRL3203, a highly expressed gene (tcrC) was detected in a high titer producing mutant derived from the parental strain NRRL3203 by NTG mutagenesis. The analysis of the nucleotide sequence of the 2. 8-kb BamHI fragment containing tcrC gene showed that the predicted tcrC gene product is a protein consisting of 512 amino acids. The deduced amino acid sequence had a high level identity with that of the self-defense gene (tet347) of Streptomyces rimosus, known to mediate oxytetracycline efflux. The tcrC gene-inactivated strains generated from strain NRRL3203 by gene replacement had a 90% decrease in the level of resistance to tetracycline and the antibiotic productivity when compared with the parental strain.

• 社団法人日本農芸化学会の論文
• 1995-10-23

著者

• AISAKA KAZUO

  Tokyo Research Laboratories, Kyowa Hakko Kogyo Co.Ltd.

• Aisaka K

  Biofrontier Laboratories Kyowa Hakko Kogyo Co. Ltd.

• Aisaka K

  Japan Tobacco Inc. Osaka Jpn

• Aisaka Kazuo

  Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.

• KATSUMATA RYOICHI

  Tokyo Research Laboratories, Kyowa Hakko Kogyo Co.Ltd.

• HASEGAWA Mamoru

  Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.

• Hasegawa Mamoru

  Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.

• Dairi Tohru

  Tokyo Research Laboratories of Kyowa Hakko Kogyo Co., Ltd.

• Katsumata R

  Graduate School Of Agricultural Science Tohoku University

• Katsumata Ryoichi

  Tokyo Researach Laboratories Kyowa Hakko Kogyo Co. Ltd.

• Dairi T

  Biotechnology Res. Center Toyama Prefectural Univ.

• Dairi Tohru

  Hokkaido Univ. Hokkaido Jpn

• Hasegawa Morifumi

  Laboratory of Agricultural Chemicals, Faculty of Agriculture, Ibaraki University

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