Enzymatic Synthesis of Novel Disaccharides Using Disaccharide Phosphorylases
スポンサーリンク
概要
- 論文の詳細を見る
A method for the synthesis of novel disaccharides was developed. It involved the following two steps. The first step consisted of two continuous reactions : the conversion of maltose to β-_D-glucose-1-phosphate and _D-glucose by the phosphorolytic activity of maltose phosphorylase and the specific consumption of only _D-glucose by incubation with glucose-consuming yeast cells. The second step involved the addition of an appropriate carbohydrate and its condensation with the remaining β-_D-glucose-1-phosphate by the synthetic activity of maltose phosphorylase or trehalose phosphorylase. Several factors affecting the yields of disaccharides were optimized. Using this method, five maltose-like derivatives and two trehalose-like derivatives were synthesized from maltose and the corresponding carbohydrates. Among these, 4-O-α-_D-glucopyranosyl-_L-fucopyranose (Glc(α1-4)Fuc) and α-_D-glucopyranosyl α-_D-fucopyranoside (Glc(α1-1α)Fuc) were purified, and identified by ^1H NMR and ^<13>C NMR.
- 社団法人日本生物工学会の論文
- 2000-08-25
著者
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Masuda Tomomi
Tokyo Research Laboratories Kyowa Hakko Kogyo Co.ltd.
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UOSAKI Youichi
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
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Uosaki Y
Kyowa Hakko Kogyo Co. Ltd. Tokyo Jpn
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Uosaki Youichi
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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AISAKA KAZUO
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co.Ltd.
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MASUDA-KATO TOMOMI
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co.Ltd.
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CHIKAMUNE TADASHI
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co.Ltd.
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KAMITORI KAZUYO
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co.Ltd.
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SAITO YUTAKA
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co.Ltd.
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Aisaka K
Biofrontier Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Aisaka K
Japan Tobacco Inc. Osaka Jpn
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Aisaka Kazuo
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Kamitori Kazuyo
Tokyo Research Laboratories Kyowa Hakko Kogyo Co.ltd.
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Chikamune Tadashi
Tokyo Research Laboratories Kyowa Hakko Kogyo Co.ltd.
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