Purification and Characterization of Cytosolic Serine Hydroxymethyltransferase from Euglena gracilis z(Biological Chemistry)
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概要
- 論文の詳細を見る
Cytosolic seine hydroxymethyltransferase [L-serine : tetrahydrofolate 5,10-hydroxymethyltransferase, EC 2.1.2.1] from Euglena gracilis z purified by precipitation with ammonium sulfate followed by column chromatography on DEAE-cellulose, and by affinity chromatography on an L-adsorbent. Through this procedure the cytosolic enzyme (s-SHMT) was purified 420-fold with a yield of more than 26.8%. The preparation gave a single band with an M_r of 45,000 on SDS-PAGE. The native enzyme contained 2 mol of pyridoxal phosphate/mol of enzyme, and had an M_r of 90,000 on gel filtration, indicating a dimeric structure. Several other properties of the enzyme were also studied.
- 社団法人日本農芸化学会の論文
- 1991-09-23
著者
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NAKANO Yoshihisa
Department of Applied Biological Chemistry, Graduate School of Life and Environmental Sciences, Osak
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Masuda Tomomi
Tokyo Research Laboratories Kyowa Hakko Kogyo Co.ltd.
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NAKANO Yoshihisa
Graduate School of Life and Environmental Sciences of Osaka Prefecture University
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KITAOKA Shozaburo
Department of Agricultural Chemistry, University of Osaka Prefecture
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Nakano Yoshihisa
Department Of Agriculture University Of Osaka Prefecture
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SAKAMOTO Masako
Shitennoji International Buddhist University
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MASUDA Tsutomu
Shitennoji International Buddhist University
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YANAGIMOTO Yukio
Shitennoji International Buddhist University
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Kitaoka S
Univ. Osaka Prefecture Osaka Jpn
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Kitaoka Shozaburo
Department Of Agricultural Chemistry
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Masuda T
Noda Inst. Scientific Res. Chiba Jpn
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Sakamoto M
Osaka Univ. Medical School Osaka
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Nakano Yoshihisa
Department Of Agricultural Chemistry
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