Flow Cytometric Estimation of Cytotoxic Activity of Rhodexin A Isolated from Rhodea japonica in Human Leukemia K562 Cells

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概要

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• We have examined the cytotoxic effect of rhodexin A isolated from the extract of Rhodea japonica on human leukemia K562 cells using a flow cytometer and compared it with that of ouabain. Rhodexin A at 30 n_M started to attenuate growth without affecting viability and further increases in the concentration of rhodexin A (100 n_M or more) completely inhibited growth with decreasing viability. Rhodexin A at 30-100 nM increased the G_2M population, but decreased the G_0G_1, population, suggesting cell cycle arrest in the G_2M phase. Rhodexin A at 100n_M increased the number of cells with hypodiploid DNA, indicating that rhodexin A induced apoptosis. The potency of rhodexin A to inhibit growth was greater than that of ouabain. The results indicate that rhodexin A exerts a potent inhibitory action on the growth of human leukemia KS62 cells by inducing cell cycle arrest and apoptosis. Rhodexin A may also be a candidate for cancer treatment because there have been clinical reports of tumor regression in patients taking cardiac glycosides.

• 公益社団法人日本薬学会の論文
• 2003-05-01

著者

• Masuda Tomomi

  Tokyo Research Laboratories Kyowa Hakko Kogyo Co.ltd.

• Masuda T

  Faculty Of Integrated Arts And Sciences The University Of Tokushima

• Masuda Toshiya

  Laboratory Of Organic Chemistry Faculty Of Agriculture Nagoya University

• Masuda Toshiya

  Laboratories Of Cellular Signaling And Bioorganic Chemistry Faculty Of Integrated Arts And Sciences

• Masuda Toshiya

  Faculty Of Human Life Science Osaka City University

• Masuda Toshiya

  Faculty Of Integrated Arts And Sciences The University Of Tokushima

• Masuda T

  Laboratories Of Cellular Signaling And Bioorganic Chemistry Faculty Of Integrated Arts And Sciences

• OYAMA Yasuo

  Laboratories of Cellular Signaling and Bioorganic Chemistry, Faculty of Integrated Arts and Sciences

• YAMAMOTO Natsuko

  Faculty of Integrated Arts and Sciences, University of Tokushima

• TOI Yukiko

  Faculty of Integrated Arts and Sciences, University of Tokushima

• Kanemaru Kaori

  徳島大学 総合科学部生物有機化学

• Kanemaru Kaori

  Laboratories Of Cellular Signaling And Bioorganic Chemistry Faculty Of Integrated Arts And Sciences

• Kanemaru Kaori

  Laboratory Of Cellular Signalling Faculty Of Integrated Arts And Sciences The University Of Tokushim

• Yamamoto N

  Calpis Co. Ltd. Kanagawa Jpn

• Chikahisa-muramatsu Lumi

  Taiho Pharmaceutical Co. Ltd.

• Toi Yukiko

  Faculty Of Integrated Arts And Sciences University Of Tokushima

• Oyama Y

  Univ. Tokushima Tokushima Jpn

• Oyama Yasuo

  Laboratory Of Cellular Signalling Faculty Of Integrated Arts And Sciences The University Of Tokushim

• Oyama Yasuo

  Laboratories Of Cellular Signaling And Bioorganic Chemistry Faculty Of Integrated Arts And Sciences

• Nakao Hiromi

  Laboratories Of Cell Signaling And Bioorganic Chemistry Faculty Of Integrated Arts And Sciences The

• UMEBAYASHI Chisato

  Laboratory of Cell Signaling, Faculty of Integrated Arts and Sciences, The University of Tokushima

• YAMAMOTO Natsuko

  Laboratories of Cell Signaling and Bioorganic Chemistry, Faculty of Integrated Arts and Sciences, Th

• TOI Yukiko

  Laboratories of Cell Signaling and Bioorganic Chemistry, Faculty of Integrated Arts and Sciences, Th

• Oyama Y

  Laboratories Of Cell Signaling And Bioorganic Chemistry Faculty Of Integrated Arts And Sciences The

• Yamamoto N

  Faculty Of Integrated Arts And Sciences University Of Tokushima

• Umebayashi Chisato

  Laboratories Of Cell Signaling And Bioorganic Chemistry Faculty Of Integrated Arts And Sciences The

• Kanemaru K

  Laboratory of Cellular Signaling, Faculty of Integrated Arts and Sciences, The University of Tokushima

• Toi Y

  Laboratories of Cell Signaling and Bioorganic Chemistry, Faculty of Integrated Arts and Sciences, The University of Tokushima

• Chikahisa-Muramatsu L

  Taiho Pharmaceutical Co. Ltd.

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