Molecular Cloning, Functional Expression and Characterization of d-Limonene Synthase from Agastache rugosa
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概要
- 論文の詳細を見る
We cloned the gene of d-limonene synthase (ArLMS) from Agastache rugosa (Labiatae). The function of ArLMS was elucidated by the preparation of recombinant protein and subsequent enzyme assay. ArLMS consisted of 2077 nucleotides including 1839 bp of coding sequence that encodes a protein of 613 amino acids. This protein has a 60 kDa molecular weight, which is identical to that of d-limonene synthase from Schizonepeta tenuifolia (Labiatae). The deduced amino acid sequence of ArLMS shows high homology with the known d- and l-limonene synthases from Labiatae plants. Here, we discussed the amino acid residues responsible for the stereo-chemical regulation in limonene biosynthesis.
- 公益社団法人日本薬学会の論文
- 2002-05-01
著者
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HONDA Gisho
Department of Pharmaceutical Health Care, Faculty of Pharmaceutical Sciences, Himeji Dokkyo Universi
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Ito Michiho
Department Of Pharmacognosy Graduate School Of Pharmaceutical Science Kyoto University
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Ito M
Graduate School Of Pharmaceutical Sciences Kyoto University
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Ito M
Department Of Pharmacognosy Graduate School Of Pharmaceutical Sciences Kyoto University
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Ito Michiho
北里大学 薬学部
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Masuda T
Faculty Of Integrated Arts And Sciences The University Of Tokushima
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Masuda Toshiya
Faculty Of Human Life Science Osaka City University
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Masuda Toshiya
Faculty Of Integrated Arts And Sciences The University Of Tokushima
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Maruyama Takuro
Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyoto University
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Saeki Daisuke
Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyoto University
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Honda Gisho
Department Of Pharmacognosy Graduate School Of Pharmaceutical Science Kyoto University
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Honda Gisho
Department Of Pharmaceutical Health Care Faculty Of Pharmaceutical Sciences Himeji Dokkyo University
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Saeki Daisuke
Department Of Pharmacognosy Graduate School Of Pharmaceutical Sciences Kyoto University
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Honda G
Graduate School Of Pharmaceutical Sciences Kyoto University
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Ito Michio
Department of Biology, Faculty of Science, Nagoya University
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