In vivo Genotoxic Potential of Kojic Acid in Rodent Multiple Organs Detected by the Comet Assay
スポンサーリンク
概要
- 論文の詳細を見る
Kojic acid has been used for skin whitening as a cosmetic agent. Kojic acid is believed to be hepatocarcinogenic in mice. We conducted the comet assay in mouse and rat multiple organs to evaluate its in vivo genotoxic potential. Kojic acid induced dose-dependent DNA damage in ddY mouse stomach and liver and in Wistar rat stomach, liver, lung, and bone marrow after a single gavage administration at ≦ 1000 mg/kg. Its hepatic genotoxicity detected by the comet assay seems to contradict to absence of its hepatic tumor initiating activity revealed by the two-step carcinogenesis studies with mice and rats. However, considering the possibility that the sensitivity of the two-step carcinogenesis studies performed are not high enough to detect weak initiating activity of a chemical, it would be premature to conclude that its hepatic genotoxicity contradicts to the absence of initiating activity. In mice fed a diet containing 3% kojic acid for up to 10 days, DNA migration increased in the stomach after feeding for 2 days but it did not increased after feeding for 1 day and a 4 days. In the colon, DNA migration after feeding of 3% kojic acid for 2 days was higher than the control values, but this increase was not statistically significant. In the stomach and colon, any statistically significant increases in DNA migration were not observed after feeding of 1.5% kojic acid. In the liver, DNA migration increased with feeding period and the increases after feeding of 3% and 1.5% kojic acid for g 4 days and 6 days, respectively, were statistically significant. Our present results suggested good correlation between hepatocarcinogenicity of kojic acid and in-creasing tendency of its hepatic genotoxicity with dosing period when it is given to mice continuously in the diet.
- 日本環境変異原学会の論文
- 2008-02-20
著者
-
SASAKI Yu
Laboratory of Genotoxicity, Faculty of Chemical and Biological Engineering, Hachinohe National Colle
-
NAKAMURA Takanori
Department of Pharmaceutical Health Care, Faculty of Pharmaceutical Sciences, Himeji Dokkyo Universi
-
HONDA Gisho
Department of Pharmaceutical Health Care, Faculty of Pharmaceutical Sciences, Himeji Dokkyo Universi
-
Sasaki Yu
Laboratory Of Genotoxicity Faculty Of Chemical And Biological Engineering Hachinohe National College
-
Yokohama Natsue
Laboratory Of Genotoxicity Faculty Of Chemical And Biological Engineering Hachinohe National College
-
Nakamura Takanori
Department Of Pharmaceutical Health Care Faculty Of Pharmaceutical Sciences Himeji Dokkyo University
-
Kawaguchi Satomi
Laboratory Of Genotoxicity Faculty Of Chemical And Biological Engineering Hachinohe National College
-
Kawaguchi Satomi
Kobuchisawa Research Laboratories, Fuji Biomedix Co., Ltd.
-
Yokohama Natsue
Chemical Quality Evaluation Div., Canon Inc.
-
Nakamura Takanori
Department Of Chemistry Yokohama City University
-
Honda Gisho
Department Of Pharmaceutical Health Care Faculty Of Pharmaceutical Sciences Himeji Dokkyo University
関連論文
- Does p53 Status Affect the Sensitivity of the Comet Assay?
- Absence of DNA Damage in Comet Assay and Liver Initiation Activity of Cinnamaldehyde
- Tissue-Specific Expression of Fucosylated Glycosphingolipid Species in Rat Prostate
- Investigation on liver initiation activity and DNA damages of flumequine in mice.(GENERAL SESSION BY ORAL PRESENTATION)(CARCINOGENICITY)
- Differential colon DNA damage induced by azo food additives between rats and mice
- Detection of DNA Single Strand Breaks Induced by Chemical Mutagens Using the Acellular Comet Assay
- O-13 Genotoxic potential in vitro and liver initiation activity of norfloxacin(CARCINOGENICITY)(GENERAL SESSION BY ORAL PRESENTATION)(Proceedings of the 31st Annual Meeting)
- 石英ガラスにおける形状回復効果
- バイセラミックスガラスの形状記憶効果
- 準結晶が分散したAl-Li-Cu系合金の脆性評価
- Induction of Cell Adhesion by Galectin-8 and its Target Molecules in Jurkat T-Cells
- Characterization of Galectin-9-Induced Death of Jurkat T Cells
- Experimental and Theoretical (AM1) Studies on Photoaddition Reactions of 3-Nitro-2-enopyranoside Derivatives
- ABSENCE OF IN VIVO GENOTOXICITY AND LIVER INITIATION ACTIVITY OF DICYCLANIL
- Quantification of Galectin-7 and Its Localization in Adult Mouse Tissues
- Perilla citriodora from Taiwan and Its Phytochemical Characteristics
- Four New 2-(2-Phenylethyl)chromone Derivatives from Withered Wood of Aquilaria sinensis
- Suppressive effects of Sairei-to on mesangial proliferation in a rat model of glomerulonephritis
- Expression pattern and role of Galectin1 during early mouse myogenesis
- Suppressive Effects of Perilla frutescens on Mesangioproliferative Glomerulonephritis in Rats
- In vivo Genotoxic Potential of Kojic Acid in Rodent Multiple Organs Detected by the Comet Assay
- Evaluation of in vitro Genotoxic Potential of Kojic Acid in Human Lymphoblastoid Cells
- Sedative effects of vapor inhalation of agarwood oil and spikenard extract and identification of their active components
- Gentic Analysis of Nothoapiol Formation in Perilla frutescens
- The comet assay with eight mouse organs : Results with currently used food dyes by single and multiple treatment.(GENERAL SESSION BY ORAL PRESENTATION)(GENOTOXICITY)
- Molecular Cloning, Functional Expression and Characterization of d-Limonene Synthase from Agastache rugosa
- Molecular Cloning, Functional Expression and Characterization of(E)-β-Farnesene Synthase from Citrus junos
- Onset of Three-Dimensional Thermal Convection in a Rectangular Parallelepiped Cavity
- マルエージング鋼の超音波熱処理
- Onset of Three-Dimensional Thermal Convection in a Rectangular Parallelepiped Cavity
- Repulsion of Eigenvalues in the Rayleigh-Benard Problem
- Tissue-Specific Expression of Fucosylated Glycosphingolipid Species in Rat Prostate
- Genotoxicity-suppressing Effect of Aqueous Extract of Connarus ruber Cortex
- How Is the Formation of Comet Tails Supported by the Incision Step of Nucleotide Excision Repair?