Molecular Cloning, Functional Expression and Characterization of(E)-β-Farnesene Synthase from Citrus junos
スポンサーリンク
概要
- 論文の詳細を見る
we cloned the gene of the acyclic sesquiterpene synthase, (E)-β-farnesene synthase(CJFS)from Yuzu(Cit-rus junos, Rutaceae). The function of CJFS was elucidated by the preparation of recombinant protein and subsequent enzyme assay. CJFS consisted of 1867 nucleotides including 1680 bp of coding Sequence encoding a protein of 560 amino acids with a molecular weight of 62 kDa. The deduced amino acid sequence possessed characteristic amino acid residues, such as the DDxxD motif, which are highly conserved among terpene synthases. This is the first report of the cloning of a terpene synthase from a Rutaceous plant. A possible reaction mechanism for terpene biosynthesis is also discussed on the basis of sequence comparison of CJFS with known sesquiterpene synthase genes.
- 公益社団法人日本薬学会の論文
- 2001-10-01
著者
-
HONDA Gisho
Department of Pharmaceutical Health Care, Faculty of Pharmaceutical Sciences, Himeji Dokkyo Universi
-
Ito Michiho
Department Of Pharmacognosy Graduate School Of Pharmaceutical Science Kyoto University
-
Ito M
Graduate School Of Pharmaceutical Sciences Kyoto University
-
Ito M
Department Of Pharmacognosy Graduate School Of Pharmaceutical Sciences Kyoto University
-
Ito Michiho
北里大学 薬学部
-
Masuda T
Faculty Of Integrated Arts And Sciences The University Of Tokushima
-
Masuda Toshiya
Faculty Of Human Life Science Osaka City University
-
Masuda Toshiya
Faculty Of Integrated Arts And Sciences The University Of Tokushima
-
Maruyama Takuro
Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyoto University
-
Honda Gisho
Department Of Pharmaceutical Health Care Faculty Of Pharmaceutical Sciences Himeji Dokkyo University
-
Honda G
Graduate School Of Pharmaceutical Sciences Kyoto University
関連論文
- Does p53 Status Affect the Sensitivity of the Comet Assay?
- Detection of DNA Single Strand Breaks Induced by Chemical Mutagens Using the Acellular Comet Assay
- Lack of Action Potential-Prolonging Effect of Terfenadine on Rabbit Myocardial Tissue Preparations(Pharmacology)
- Atria Selective Prolongation by NIP-142, an Antiarrhythmic Agent, of Refractory Period and Action Potential Duration in Guinea Pig Myocardium
- Effect of Olmesartan on Oxidative Stress in Hemodialysis Patients
- Oxidation and Carboxy Methyl Lysine-Modification of Albumin : Possible Involvement in the Progression of Oxidative Stress in Hemodialysis Patients
- New Sesquiterpene Hydroperoxides with Trypanocidal Activity from Pogostemon cablin
- New Norditerpenoids with Trypanocidal Activity from Vitex trifolia
- Trypanocidal Flavonoids from Sophora flavescens
- Flavonoid Constituents of Dracocephalum kotschyi Growing in Iran and Their Trypanocidal Activity
- Screening of Natural Medicines Used in Vietnam for Trypanocidal Activity against Epimastigotes of Trypanosoma cruzi
- Perilla citriodora from Taiwan and Its Phytochemical Characteristics
- Anticarcinogenic compounds in the Uzbek medicinal plant, Helichrysum maracandicum
- A new phenolic glucoside from an Uzbek medicinal plant, Origanum tyttanthum
- P-519 ABSOLUTE STEREOCHEMISTRY OF CHROMONE DERIVATIVES FROM AGARWOOD
- Two New Monoterpene Glycosides and Trypanocidal Terpenoids from Dracocephalum kotschyi
- Field Survey of Cinnamon in Viet Nam
- Phenylethanoid Glycosides Targeting Mammalian DNA Polymerases
- Four New 2-(2-Phenylethyl)chromone Derivatives from Withered Wood of Aquilaria sinensis
- A New Megastigmane Glucoside from Phlomis spinidens
- Molecular Cloning, Functional Expression and Characterization of d-Limonene Synthase from Schizonepeta tenuifolia
- Radical and Superoxide Scavenging Activities of Matairesinol and Oxidized Matairesinol
- Suppressive effects of Sairei-to on mesangial proliferation in a rat model of glomerulonephritis
- Glycosidic constituents of the leaves of an Okinawan Leguminosae plant, Derris trifoliata Lour
- Chemical constituents of an Uzbek medicinal plant, Perovskia scrophularifolia
- Indole alkaloids from Rheum maximowiczii
- Sesquiterpenoids from Artemisia tenuisecta
- Absolute Configuration of Volicitin from the Regurgitant of Lepidopteran Caterpillars and Biological Activity of Volicitin-Related Compounds
- Screening for Tyrosinase Inhibitors among Extracts of Seashore Plants and Identification of Potent Inhibitors from Garcinia subelliptica
- Cytotoxic Screening of Medicinal and Edible Plants in Okinawa, Japan, and Identification of the Main Toxic Constituent of Rhodea japonica (Omoto)(Organic Chemistry)
- Isolation and Antioxidant Activity of Galloyl Flavonol Glycosides from the Seashore Plant, Pemphis acidula
- Cytotoxic Effect of Drosophila Deoxynucleoside Kinase Gene on Replicating Plasmid in HeLa Cells
- Induction of Various Mutations during PCRs with Manganese and 8-Hydroxy-dGTP
- Induction of Transition and Transversion Mutations during Random Mutagenesis PCR by the Addition of 2-Hydroxy-dATP (Biochemistry/Molecular Biology)
- Suppressive Effects of Perilla frutescens on Mesangioproliferative Glomerulonephritis in Rats
- In vivo Genotoxic Potential of Kojic Acid in Rodent Multiple Organs Detected by the Comet Assay
- Evaluation of in vitro Genotoxic Potential of Kojic Acid in Human Lymphoblastoid Cells
- Evaluation of volatile components from spikenard : valerena-4,7(11)-diene is a highly active sedative compound
- Sedative effects of vapor inhalation of agarwood oil and spikenard extract and identification of their active components
- cDNA Isolation and Functional Expression of Myrcene Synthase from Perilla frutescens(Pharmacognosy)
- Field Survey of Glycyrrhiza Plants in Central Asia (3) Chemical Characterization of G. glabra Collected in Uzbekistan
- Field Survey of Glycyrrhiza Plants in Central Asia (2). : Characterization of Phenolics and Their Variation in the Leaves of Glycyrrhiza Plants Collected in Kazakhstan
- Field Survey of Glycyrrhiza Plants in Central Asia (1). Characterization of G. uralensis, G. glabra and the Putative Intermediate Collected in Kazakhstan
- Phlomisflavosides A and B, New Flavonol Bisglycosides from Phlomis spinidens
- Chemical Constituents of Prangos tschimganica ; Structure Elucidation and Absolute Configuration of Coumarin and Furanocoumarin Derivatives with Anti-HIV Activity
- Farnesyl Hydroxybenzoic Acid Derivatives from Ferula kuhistanica
- Secoiridoid Glycosides from Gentiana olivieri
- Gentic Analysis of Nothoapiol Formation in Perilla frutescens
- Chemical Composition of the Essential oil of Perilla frutescens
- Antioxidant Mechanism Studies on Ferulic Acid : Isolation and Structure Identification of the Main Antioxidation Product from Methyl Ferulate
- Molecular Cloning, Functional Expression and Characterization of d-Limonene Synthase from Agastache rugosa
- Molecular Cloning, Functional Expression and Characterization of(E)-β-Farnesene Synthase from Citrus junos
- Influence of composition upon the variety of tastes in Cinnamomi Cortex
- Anti-ulcer Effects of Antioxidants, Quercetin, a-Tocopherol, Nifedipine and Tetracycline in Rats
- Simultaneous Evaluation of Gastric and Duodenal Ulcers-Healing Activities of Anti-ulcer Agents in Rats
- Healing-Promoting Action of the Zinc-Cimetidine Complex on Acetic Acid-Induced Gastric Ulcers in Rats
- Low Molecular Weight Chitosan Prevents the Progression of Low Dose Streptozotocin-Induced Slowly Progressive Diabetes Mellitus in Mice
- New Model of Progressive Non-Insulin-Dependent Diabetes Mellitus In Mice Induced by Streptozotocin
- Flow Cytometric Estimation of Cytotoxic Activity of Rhodexin A Isolated from Rhodea japonica in Human Leukemia K562 Cells
- Characterization of Ligand Binding Sites on the α_1-Acid Glycoprotein in Humans, Bovines and Dogs
- Tyrosinase Inhibitory Activity of Ethanol Extracts from Medicinal and Edible Plants Cultivated in Okinawa and Identification of a Water-Soluble Inhibitor from the Leaves of Nandina domestica
- Cytotoxic Activity of Maytanprine Isolated from Maytenus diversifolia in Human Leukemia K562 Cells(Toxicology)
- New Sesquiterpene Glucosides from Crepidiastrum lanceolatum
- Cytometric Analysis of the Cytotoxic Action of Adenosine 5'-Monophosphate on Rat Thymocytes
- New Megastigmane and Tetraketide from the Leaves of Euscaphis japonica
- An Iridoid Glucoside Dimer and a Non-glycosidic Iridoid from the Leaves of Lasianthus wallichii
- Labdane Diterpenoids from Marrubium globosum ssp. globosum
- Nepetaracemosides A and B, Iridoid Glucosides from Nepeta racemosa
- Blockade by NIP-142, an Antiarrhythmic Agent, of Carbachol-Induced Atrial Action Potential Shortening and GIRK1/4 Channel
- Studies on perilla, agarwood, and cinnamon through a combination of fieldwork and laboratory work
- Production of agarwood fragrant constituents in Aquilaria calli and cell suspension cultures
- An Efficient Synthesis of (±)-Hasakol, a Bioactive Coumarin from Citrus hassaku
- Molecular Recognition of Deoxycholic Acids by Pyrene-Appended γ-Cyclodextrin Connected with a Rigid Azacrown Spacer
- Identification of the Antioxidation Reaction Products from a Sinapic Ester in a Lipid Oxidation System
- Tyrosinase Inhibitory Activity of Ethanol Extracts from Medicinal and Edible Plants Cultivated in Okinawa and Identification of a Water-Soluble Inhibitor from the Leaves of Nandina domestica
- Receptor-Mediated Uptake of Human α1-Acid Glycoprotein into Liver Parenchymal Cells in Mice
- Antioxidant Activity of Butane Type Lignans, Secoisolariciresinol, Dihydroguaiaretic Acid, and 7,7'-Oxodihydroguaiaretic Acid
- Synthesis and Antioxidant Activity of Olivil-Type Lignans
- Synthesis and Antioxidant Activity of Oxygenated Furofuran Lignans
- Construction of an Expression System for Human α_1-Acid Glycoprotein in E. coli : the Roles of Oligosaccharide Moieties in Structural and Functional Properties
- Germination rates of perilla (Perilla frutescens (L.) Britton) mericarps stored at 4℃ for 1-20 years
- PB103 TOTAL SYNTHESIS OF A TUMOR PROMOTER, TELEOCIDIN B, BY DIRECT INTRODUCTION OF FUNCTIONAL GROUPS ON THE BENZENE PART OF AN INDOLE DERIVATIVE
- Guest-Induced Excimer Formation of β-Cyclodextrin Modified with a Branched Arm Possessing Two Naphthyl Moieties
- Concise Synthesis of Valerena-4,7(11)-diene, a Highly Active Sedative, from Valerenic Acid
- Indole alkaloids from Rheum maximowiczii
- Sesquiterpenoids from Artemisia tenuisecta
- A New Megastigmane Glucoside from Phlomis spinidens
- Screening of Natural Medicines Used in Vietnam for Trypanocidal Activity against Epimastigotes of Trypanosoma cruzi
- Field Survey of Cinnamon in Viet Nam
- Trypanocidal Flavonoids from Sophora flavescens
- Flavonoid Constituents of Dracocephalum kotschyi Growing in Iran and Their Trypanocidal Activity
- Phenylethanoid Glycosides Targeting Mammalian DNA Polymerases
- Essential Oil Composition of Hybrids and Amphidiploids of Japanese Wild Perilla
- Sedative effects of vapor inhalation of the essential oil of Microtoena patchoulii and its related compounds
- New Sesquiterpene Glucosides from Crepidiastrum lanceolatum
- A New Phenylpropanoid-Glycerol Conjugate from Helitiera littoralis Dryand
- Folk Medicines in Dan Phu-o-ng Village in Southern Vietnam
- Sedative Effects of Inhaled Benzylacetone and Structural Features Contributing to Its Activity
- Genomic organization of δ-guaiene synthase genes in Aquilaria crassna and its possible use for the identification of Aquilaria species
- Locomotor-Reducing Effects and Structural Characteristics of Inhaled Zerumbone and Tetrahydrozerumbone Derivatives