Cytometric Analysis of the Cytotoxic Action of Adenosine 5'-Monophosphate on Rat Thymocytes
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概要
- 論文の詳細を見る
The cascade of adenosine 5'-monophosphate-activated protein kinase (AMPK) is known to be a sensor of cellular energy charge. In this context, a paradigm for obesity treatment via the activation of AMPK has attracted the suppliers of complementary medicines and supplements. It is possible that products that increase the concentration of adenosine 5'-monophosphate (AMP) in the body would be efficacious in reducing body weight. However, since there is currently little information concerning the toxicity of AMP, the cytotoxic action of AMP on rat thymocytes was examined by flow cytometry. Incubation of cells with AMP at a concentration of 30μM or more for 24hr significantly increased the populations of dead cells, shrunken cells, and cells containing hypodiploidal DNA in a concentration-dependent manner. Z-VAD-FMK, a pan-inhibitor of caspases, attenuated the AMP-induced changes in cell populations. It is concluded that AMP at a concentration of 30μM or more exerts a cytotoxic action, which is dependent on the activation of caspases.
- 公益社団法人日本薬学会の論文
- 2008-12-01
著者
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Masuda T
Faculty Of Integrated Arts And Sciences The University Of Tokushima
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Masuda Toshiya
Laboratory Of Organic Chemistry Faculty Of Agriculture Nagoya University
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Masuda Toshiya
Laboratories Of Cellular Signaling And Bioorganic Chemistry Faculty Of Integrated Arts And Sciences
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Masuda Toshiya
Faculty Of Human Life Science Osaka City University
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Masuda Toshiya
Faculty Of Integrated Arts And Sciences The University Of Tokushima
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OYAMA Yasuo
Laboratories of Cellular Signaling and Bioorganic Chemistry, Faculty of Integrated Arts and Sciences
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Kanemaru Kaori
徳島大学 総合科学部生物有機化学
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Kanemaru Kaori
Laboratories Of Cellular Signaling And Bioorganic Chemistry Faculty Of Integrated Arts And Sciences
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Kanemaru Kaori
Laboratory Of Cellular Signalling Faculty Of Integrated Arts And Sciences The University Of Tokushim
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Oyama Y
Univ. Tokushima Tokushima Jpn
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Oyama Yasuo
Laboratory Of Cellular Signalling Faculty Of Integrated Arts And Sciences The University Of Tokushim
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Oyama Yasuo
Laboratories Of Cellular Signaling And Bioorganic Chemistry Faculty Of Integrated Arts And Sciences
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Sakanashi Yoko
Laboratories of Cellular Signaling and Bioorganic Chemistry, Faculty of Integrated Arts and Sciences
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Morimoto Midori
Laboratories of Cellular Signaling and Bioorganic Chemistry, Faculty of Integrated Arts and Sciences
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Nishimura Yumiko
Okayama University Dental School
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Matsui Hiroko
Faculty of Dental Science, Kyushu University
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Sakanashi Yoko
Univ. Tokushima Tokushima Jpn
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Morimoto Midori
Laboratories Of Cellular Signaling And Bioorganic Chemistry Faculty Of Integrated Arts And Sciences
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Matsui Hiroko
Faculty Of Dental Science Kyushu University
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Sakanasi Yoko
Laboratories of Cellular Signaling and Bioorganic Chemistry, Faculty of Integrated Arts and Sciences, The University of Tokushima
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Oyama Yasuo
Laboratories for Pharmacology, The University of Tokushima
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