Effect of econazole on membrane calcium transport in rat thymocytes
スポンサーリンク
概要
- 論文の詳細を見る
Econazole, one of azole antifungals, is proven to exhibit an inhibitory action on Mycobateriumtuberculosis and its multidrug-resistant strains under in vitro and ex vivo conditions. However, econazole has been used as a pharmacological tool for inhibiting capacitative Ca2+ influx and exerts multiple effects on cellular Ca2+ circumstance. Therefore, to suggest the toxic effect of econazole at therapeutic concentrations, we have tested on the effect on membrane Ca2+ transport in rat thymocytes by using a flow cytometer with Fluo-3, an indicator of intracellular Ca2+. Econazole at concentrations of 1-3 μM increased membrane Ca2+ permeability and inhibited capacitative Ca2+ influx without affecting passive Ca2+ influx, Ca2+ release from intracellular Ca2+store sites, and membrane Ca2+ pump. Econazole at 0.3 μM, a therapeutic concentration against tuberculosis caused by multidrug-resistant and latent M. tuberculosis, did not affect membrane Ca2+ transport. It may be suggested that econazole at therapeutic concentrations exerts no side effect related to Ca2+.
- 徳島大学の論文
著者
-
Oyama Yasuo
Laboratories Of Cellular Signaling And Bioorganic Chemistry Faculty Of Integrated Arts And Sciences
-
Sakanashi Yoko
Laboratories of Cellular Signaling and Bioorganic Chemistry, Faculty of Integrated Arts and Sciences
-
Oyama Yasuo
Univ. Tokushima Tokushima Jpn
-
Hirata Yoshiko
Laboratory Of Cellular Signaling Faculty Of Integrated Arts And Sciences The University Of Tokushima
-
Sakanashi Yoko
Laboratory Of Cellular Signaling Faculty Of Integrated Arts And Sciences The University Of Tokushima
-
Akiyama Jun
Laboratory Of Cellular Signaling Faculty Of Integrated Arts And Sciences The University Of Tokushima
-
Ogasa Hiroshi
Laboratory of Cellular Signaling, Faculty of Integrated Arts and Sciences, The University of Tokushi
-
Yamashita Kohei
Laboratory of Cellular Signaling, Faculty of Integrated Arts and Sciences, The University of Tokushi
-
Shibagaki Haruka
Laboratory of Cellular Signaling, Faculty of Integrated Arts and Sciences, The University of Tokushi
-
Tanaka Fumika
Laboratory of Cellular Signaling, Faculty of Integrated Arts and Sciences, The University of Tokushi
-
Tanaka Fumika
Laboratory Of Cellular Signaling Faculty Of Integrated Arts And Sciences The University Of Tokushima
-
Ogasa Hiroshi
Laboratory Of Cellular Signaling Faculty Of Integrated Arts And Sciences The University Of Tokushima
-
Yamashita Kohei
Laboratory Of Cellular Signaling Faculty Of Integrated Arts And Sciences The University Of Tokushima
-
Shibagaki Haruka
Laboratory Of Cellular Signaling Faculty Of Integrated Arts And Sciences The University Of Tokushima
-
Oyama Yasuo
Laboratories for Pharmacology, The University of Tokushima
関連論文
- New Curcuminoids Isolated from Zingiber cassumunar Protect Cells Suffering from Oxidative Stress: A Flow-Cytometric Study Using Rat Thymocytes and H_2O_2
- Cytotoxic Screening of Medicinal and Edible Plants in Okinawa, Japan, and Identification of the Main Toxic Constituent of Rhodea japonica (Omoto)(Organic Chemistry)
- Isolation and Antioxidant Activity of Galloyl Flavonol Glycosides from the Seashore Plant, Pemphis acidula
- Extract of Ginkgo biloba Leaves Attenuates Kainate-Induced Increase in Intracellular Ca^ Concentration of Rat Cerebellar Granule Neurons(Pharmacognosy)
- Effects of Triorganotins on Brain Neurons, Thymocytes, and Tumor Cells
- Cytotoxic Actions of FTY720, a Novel Immunosuppressant, on Thymocytes and Brain Neurons Dissociated From the Rat
- Oxidative Stress : Induced Increase in Intracellular Ca^ and Ca^ : Induced Increase in Oxidative Stress: An Experimental Model Using Dissociated Rat Brain Neurons
- Fluorescent Estimation of H_2O_2-Induced Changes in Cell Viability and Cellular Nonprotein Thiol Level of Dissociated Rat Thymocytes
- Flow Cytometric Analysis of the H_2O_2-Induced Increase in Intracellular Ca^ Concentration of Rat Thymocytes
- Change in Membrane Permeability Induced by Amyloid β- Protein Fragment 25-35 in Brain Neurons Dissociated from Rats
- Formaldehyde-Induced Shrinkage of Rat Thymocytes
- Flow Cytometric Estimation of Cytotoxic Activity of Rhodexin A Isolated from Rhodea japonica in Human Leukemia K562 Cells
- Cytotoxic Activity of Maytanprine Isolated from Maytenus diversifolia in Human Leukemia K562 Cells(Toxicology)
- Cytometric Analysis of the Cytotoxic Action of Adenosine 5'-Monophosphate on Rat Thymocytes
- Effects of Zn^ chelators, DTPA and TPEN, and ZnCl_2 on the cells treated with hydrogen peroxide: a flow-cytometric study using rat thymocytes
- Cytotoxic actions of lidocaine at sublethal concentrations : A model in vitro experiment using rat thymocytes
- ZnCl_2 and vitamin C, known as antioxidants, differently potentiate the cytotoxicity of H_2O_2 in rat thymocytes : Cytometric analysis using forward and side scatters
- Nitroprusside increases intracellular Zn^ concentration without affecting cellular thiol content : A model experiment using rat thymocytes and FluoZin-3
- Effect of econazole on membrane calcium transport in rat thymocytes
- NOR-3, a donor of nitric oxide, increases intracellular Zn2+ concentration and decreases cellular thiol content: A model experiment using rat thymocytes, FluoZin-3, and 5-chloromethylfluorescein
- Tetracaine decreases intracellular Zn2+ concentration by inhibiting Zn2+ influx in rat thymocytes
- Flow Cytometric Estimation of the Effect of Ginkgo Biloba Extract on the Content of Hydrogen Peroxide in Dissociated Mammalian Brain Neurons.