Flow Cytometric Estimation of the Effect of Ginkgo Biloba Extract on the Content of Hydrogen Peroxide in Dissociated Mammalian Brain Neurons.
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概要
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The effect of <I>Ginkgo biloba</I> extract (GBE) on the content of hydrogen peroxide was estimated in cerebellar neurons dissociated from rats, by means of a flow-cytometer and 2'', 7''-dichloro-fluorescin (DCF) diacetate, a fluorescent dye for intracellular hydrogen peroxide. The GBE started to reduce the DCF fluorescence of the neuron at 0.1 μg/ml to 0.3 μg/ml. Further increases in the GBE concentration (up to 3 μg/ml) produced a dose-dependent decrease in the DCF fluorescence, suggesting that GBE reduces the content of hydrogen peroxide or suppresses the reactive oxygen species (ROS) formation of cerebellar neurons. The present technique may be useful for preliminary evaluations of agents affecting the ROS formation in mammalian brain neurons.
- 公益社団法人 日本薬理学会の論文
著者
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Oyama Yasuo
Laboratories Of Cellular Signaling And Bioorganic Chemistry Faculty Of Integrated Arts And Sciences
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UEHA Toshiko
Laboratory of Cell Signaling (Pharmacology), Faculty of Integrated Arts and Sciences, The University
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Noda Katsuhiko
Food Science, Faculty of Integrated Arts and Sciences, The University of Tokushima
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Hayashi Akemi
Laboratories for Pharmacology, The University of Tokushima
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Chikahisa Lumi
Taiho Pharmaceutical Co., Ltd.
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Ueha Toshiko
Laboratories for Pharmacology, The University of Tokushima
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Oyama Yasuo
Laboratories for Pharmacology, The University of Tokushima
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