Extract of Ginkgo biloba Leaves Attenuates Kainate-Induced Increase in Intracellular Ca^<2+> Concentration of Rat Cerebellar Granule Neurons(Pharmacognosy)
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概要
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In order to reveal one of possible mechanisms for neuronal protective action of extract of Ginkgo biloba leaves (EGBL), the effect of EGBL on kainate- and KCl-induced increases in intracellular Ca^<2+> concentration ([Ca^<2+>]i) of rat cerebellar neurons was examined using a confocal laser microscope with appropriate fluorescent probes. EGBL at 3μg/ml started to attenuate kainate-induced increase of [Ca^<2+>]i and further increase in EGBL concentration (up to 30μg/ml) concentration-dependently and significantly inhibited the kainate response. The complete inhibition by EGBL was observed in some neurons when the concentration was 10-30μg/ml. The kainate-induced increase in [Ca^<2+>]i was mainly due to Ca^<2+> influx through voltage-dependent Ca^<2+> channel opened by membrane depolarization via activation of kainate receptor-channel. However, the increase in [Ca^<2+>]i by KCl was not significantly affected by EGBL at concentrations where the kainate response was greatly inhibited. EGBL consisting of flavone glycosides and terpene lactones is known to be an antioxidant. Furthermore, in this study, it is shown that EGBL exerts an inhibitory action on kainate receptor (a subtype of glutamate receptor). Since some of neurodegenerative diseases are due to cell death induced by glutamate excitotoxicity and oxidative stress, EGBL may be very suitable for preventing and/or treating such diseases.
- 2005-05-01
著者
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Kobayashi M
Laboratory Of Biodynamics Graduate School Of Bioagricultural Sciences Nagoya University
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Kanada Aimi
Laboratory Of Cellular Signaling Faculty Of Integrated Arts And Sciences The University Of Tokushima
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Yamaguchi Junya
Laboratory Of Cellular Signaling Faculty Of Integrated Arts And Sciences The University Of Tokushima
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OYAMA Yasuo
Laboratories of Cellular Signaling and Bioorganic Chemistry, Faculty of Integrated Arts and Sciences
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Kanemaru Kaori
徳島大学 総合科学部生物有機化学
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Kanemaru Kaori
Laboratories Of Cellular Signaling And Bioorganic Chemistry Faculty Of Integrated Arts And Sciences
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Kanemaru Kaori
Laboratory Of Cellular Signalling Faculty Of Integrated Arts And Sciences The University Of Tokushim
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Oyama Y
Univ. Tokushima Tokushima Jpn
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Oyama Yasuo
Laboratory Of Cellular Signalling Faculty Of Integrated Arts And Sciences The University Of Tokushim
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Oyama Yasuo
Laboratories Of Cellular Signaling And Bioorganic Chemistry Faculty Of Integrated Arts And Sciences
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Oyama Y
Laboratories Of Cell Signaling And Bioorganic Chemistry Faculty Of Integrated Arts And Sciences The
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Nishimura Yumiko
Okayama University Dental School
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NISHIMURA Yumiko
Laboratory of Cellular Signaling, Faculty of Integrated Arts and Sciences, The University of Tokushi
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KOBAYASHI Masako
Laboratory of Cellular Signaling, Faculty of Integrated Arts and Sciences, The University of Tokushi
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MISHIMA Kyoko
Laboratory of Cellular Signaling, Faculty of Integrated Arts and Sciences, The University of Tokushi
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HORIMOTO Kanna
Laboratory of Cellular Signaling, Faculty of Integrated Arts and Sciences, The University of Tokushi
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Mishima Kyoko
Laboratory Of Cellular Signaling Faculty Of Integrated Arts And Sciences The University Of Tokushima
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Horimoto Kanna
Laboratory Of Cellular Signaling Faculty Of Integrated Arts And Sciences The University Of Tokushima
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Kobayashi Masako
Laboratory Of Cellular Signaling Faculty Of Integrated Arts And Sciences The University Of Tokushima
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Yamaguchi Jun-ya
Laboratory of Cellular Signaling, Faculty of Integrated Arts and Sciences, The University of Tokushima
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Mishima K
Laboratory of Cellular Signaling, Faculty of Integrated Arts and Sciences, The University of Tokushima
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Horimoto K
Laboratory of Cellular Signaling, Faculty of Integrated Arts and Sciences, The University of Tokushima
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Kanemaru K
Laboratory of Cellular Signaling, Faculty of Integrated Arts and Sciences, The University of Tokushima
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