Cytotoxic Effect of Drosophila Deoxynucleoside Kinase Gene on Replicating Plasmid in HeLa Cells
スポンサーリンク
概要
- 論文の詳細を見る
To enhance the levels of transgene expression from plasmid-based nonviral vectors, replicating plasmids containing the SV40 origin and the SV40 large T antigen gene, as a model replicating unit, were constructed. The replicating luciferase plasmid DNA produced the luciferase protein more efficiently than the non-replicating luciferase plasmid DNA, as expected. Surprisingly, the introduction of the replicating plasmid DNA containing the Drosophila melanogaster deoxyribonucleoside kinase (Dm-dNK) gene was highly cytotoxic and caused cell death without nucleoside analogs. Our results confirm that transgenes on a replicating plasmid represent an excellent tool for effective protein production and suggest that efficient production of the Dm-dNK protein in tumor cells could be an attractive cancer therapy.
- 日本薬学会の論文
著者
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Kamiya Hiroyuki
Faculty of Pharmaceutical Sciences, Hokkaido University
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Harashima Hideyoshi
Faculty of Pharmaceutical Sciences, Hokkaido University
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原島 秀吉
北大院・薬学研究科
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Ito M
Meijo Univ. Nagoya Jpn
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Ito M
Graduate School Of Pharmaceutical Sciences Kyoto University
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Ito M
Department Of Pharmacognosy Graduate School Of Pharmaceutical Sciences Kyoto University
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Shim Chang
Faculty Of Pharmaceutical Sciences University Of Tokyo : (present Address) College Of Pharmacy Seoul
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Harashima Hideyoshi
Department Of Pharmacokinetics And Pharmaceutics Graduate School Of Pharmaceutical Sciences The Univ
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Kamiya Hiroyuki
産業医科大学産業生態科学研究所
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Murata-kamiya Naoko
Inst. Indust. Ecolog. Sci. Univ. Occup. Environ. Hith.
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Ito Mana
Faculty of Pharmaceutical Sciences, Hokkaido University
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Suda Yusuke
Faculty of Pharmaceutical Sciences, Hokkaido University
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Kamiya Hiroyuki
Faculty Of Pharmaceutical Sciences Hokkaido University
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Harashima Hideyoshi
Faculty Of Pharmaceutical Science The University Of Tokushima
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Kaji Hiroshi
Inst. Indust. Ecolog. Sci. Univ. Occup. Environ. Hith.
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