Effects of non-B DNA sequences on transgene expression
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概要
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DNA conformation may be an important factor affecting gene transcription. In this study, we examined how DNA sequences with unusual conformations affect transgene expression. A30 and (CG)15 sequences that can adopt the B' and Z conformations, respectively, were introduced into a β-actin promoter. Luciferase plasmids containing the manipulated promoter were transfected into NIH3T3 cells by electroporation and were delivered into mouse livers with a hydrodynamics-based injection. Expression from plasmid with the (CG)15 sequence was multiple times higher than expression from control plasmid DNA. The A30 sequence also tended to enhance expression. These results suggest that non-B DNA sequences could improve transgene expression in cells.
- 社団法人日本生物工学会の論文
著者
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Kamiya Hiroyuki
Faculty of Pharmaceutical Sciences, Hokkaido University
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Harashima Hideyoshi
Faculty of Pharmaceutical Sciences, Hokkaido University
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Murata-kamiya Naoko
Inst. Indust. Ecolog. Sci. Univ. Occup. Environ. Hith.
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Goto Hitomi
Faculty of Pharmaceutical Sciences, Hokkaido University
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Kamiya Hiroyuki
Faculty Of Pharmaceutical Sciences Hokkaido University
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Harashima Hideyoshi
Faculty Of Pharmaceutical Science The University Of Tokushima
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Kamiya Hiroyuki
Faculty Of Pharmaceutical Sciences Hokkaido University:crest Japan Science And Technology
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Harashima Hideyoshi
Faculty of Pharmaceutical Sciences, Hokkaido University:CREST, Japan Science and Technology
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