Efficient Intradermal Delivery of Superoxide Dismutase Using a Combination of Liposomes and Iontophoresis for Protection against UV-Induced Skin Damage
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概要
- 論文の詳細を見る
Superoxide dismutase (SOD) is a potent antioxidant agent that protects against UV-induced skin damage. However, its high molecular weight is a significant obstacle for efficient delivery into the skin through the stratum corneum and development of antioxidant activity. Recently, we developed a non-invasive transfollicular delivery system for macromolecules using a combination of liposomes and iontophoresis, that represents promising technology for enhancing transdermal administration of charged drugs (iIJP/i, 403, 2011, Kajimoto iet al./i). In this study, in rats we attempted to apply this system to intradermal delivery of SOD for preventing UV-induced skin injury. SOD encapsulating in cationic liposomes was subjected to anodal iontophoresis. After iontophoretic treatment, the liposomes were diffused widely in the viable skin layer around hair follicles. In contrast, passive diffusion failed to transport liposomes efficiently into the skin. Iontophoretic delivery of liposomes encapsulating SOD caused a marked decrease in the production of oxidative products, such as malondialdehyde, hexanoyl lysine, and 8-hydroxi-2-deoxyguanosine, in UV-irradiated skin. These findings suggested that functional SOD can be delivered into the skin using a combination of iontophoresis and a liposomal system. In conclusion, we succeeded in developing an efficient intradermal SOD delivery system, that would be useful for delivery of other macromolecules.
- 公益社団法人 日本薬学会の論文
著者
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梶本 和昭
北海道大学大学院薬学研究院
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Kogure Kentaro
Department Of Biophysical Chemistry Kyoto Pharmaceutical University
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KIGASAWA Kaoru
Graduate School of Life Science, Hokkaido University
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KANAMURA Kiyoshi
TTI ellebeau Inc.
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Harashima Hideyoshi
Faculty Of Pharmaceutical Science The University Of Tokushima
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Kajimoto Kazuaki
Department Of Epidemiology Divisions Of Preventive Medicine
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Kigasawa Kaoru
Graduate School Of Life Sci. Hokkaido Univ.
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Kajimoto Kazuaki
Faculty of Pharmaceutical Sciences, Hokkaido University
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Miyashita Moeko
Graduate School of Life Science, Hokkaido University
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