Silencing of Exogenous DNA in Cultured Cells
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概要
- 論文の詳細を見る
The intranuclear disposition of exogenous DNA is highly important for the therapeutic effects of the administrated DNA. Naked luciferase-plasmid DNA was transfected into cultured cells including HeLa by electroporation, and the amounts of intranuclear plasmid DNA and luciferase were quantitated at various time points. Decrease in expression efficiency from one copy of the exogenous DNA over time occurred as the case of mouse liver, and its degrees varied between cell lines. These results suggest that exogenous DNA is 'silenced' in the cultured cells as well as in mouse hepatocytes.
- 公益社団法人日本薬学会の論文
- 2006-06-01
著者
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KAMIYA Hiroyuki
Graduate School of Pharmaceutical Sciences, Hokkaido University
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HARASHIMA Hideyoshi
Graduate School of Pharmaceutical Sciences, Hokkaido University
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OCHIAI Hiroshi
Graduate School of Pharmaceutical Sciences, Hokkaido University
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Kamiya Hiroyuki
Graduate School Of Pharmaceutical Sciences Hokkaido University
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Kamiya Hiroyuki
Faculty Of Pharmaceutical Sciences Hokkaido University:crest Japan Science And Technology
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Ochiai Hiroshi
Graduate School Of Medicine And Pharmaceutical Sciences University Of Toyama
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Ochiai Hiroshi
Faculty Of Pharmaceutical Sciences Hokkaido University
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Harashima Hideyoshi
Graduate School Of Pharmaceutical Sciences Hokkaido University:crest Japan Science And Technology Corporation (jst)
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