Delivery of Condensed DNA by Liposomal Non-viral Gene Delivery System into Nucleus of Dendritic Cells(Biopharmacy)

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概要

• 論文の詳細を見る

• In this study, we developed novel double-membranous non-viral gene delivery system modified with SV-40 T antigen-derived nuclear localization signal (NLS-DMEND) for delivery of luciferase plasmid DNA to nucleus of non-dividing mouse bone marrow-derived dendritic cells (BMDC). Intracellular trafficking and gene expression of NLS-DMEND in the BMDC were evaluated. Condensed DNA was observed in the nucleus by confocal laser scanning microscopy, and the NLS-DMEND induced significant luciferase activity in the BMDC. It was suggested that the condensed DNA particle transferred into nucleus via energy dependent manner, since the nuclear transfer was inhibited by metabolic inhibitors. In conclusion, condensed plasmid DNA was delivered into the nucleus of non-dividing BMDC by NLS-DMEND.

• 公益社団法人日本薬学会の論文
• 2006-06-01

著者

• HARASHIMA Hideyoshi

  Graduate School of Pharmaceutical Sciences, Hokkaido University

• HAMADA Hirofumi

  Department of Molecular Medicine, Sapporo Medical University School of Medicine

• AKITA Hidetaka

  Graduate School of Pharmaceutical Sciences, Hokkaido University

• Kato Kazunori

  Department Of Mechanical Engineering Shonan Institute Of Technology

• Kogure Kentaro

  Dep. Of Biophysical Chemistry Kyoto Pharmaceutical Univ.

• Akita Hidetaka

  Lab. For Molecular Design Of Pharmaceutics Fac. Of Pharmaceutical Sciences Hokkaido Univ.

• FUTAKI Shiroh

  Institute for Chemical Research, Kyoto University

• NAKAMURA Takashi

  Graduate School of Environmental Earth Science, Hokkaido University

• KOGURE Kentaro

  Graduate School of Pharmaceutical Sciences, Hokkaido University

• MORIGUCHI Rumiko

  Graduate School of Pharmaceutical Sciences, Hokkaido University

• MINOURA Arisa

  Graduate School of Pharmaceutical Sciences, Hokkaido University

• MASUDA Tomoya

  Graduate School of Pharmaceutical Sciences, Hokkaido University

• UENO Masaharu

  Faculty of Pharmaceutical Sciences, University of Toyama

• Akita Hidetaka

  Graduate School Of Pharmaceutical Sciences Hokkaido University

• Hamada Hirofumi

  Department Of Molecular Biotherapy Research Cancer Chemotherapy Canter Center Institute Japanese Fou

• Ueno Masaharu

  Faculty Of Pharmaceutical Sciences University Of Toyama

• Ueno Masaharu

  Faculty Of Phamaceutical Sciences Toyama Medical And Pharmaceutical University

• Masuda Tomoya

  Graduate School Of Pharmaceutical Sciences Hokkaido University:crest Japan Science And Technology Ag

• Minoura Arisa

  Graduate School Of Pharmaceutical Sciences Hokkaido University

• Futaki Shiroh

  Institute For Chemical Research Kyoto University

• Futaki Shiroh

  Institute For Chemical Research Kyoto University:presto Japan Science And Technology Agency (jst)

• Kato Kazunori

  Department Of Molecular Medicine Sapporo Medical University

• Kato Kazunori

  Department Of Mechanical Engineering And Science Faculty Of Engineering Tokyo Institute Of Technolog

• Moriguchi Rumiko

  Graduate School Of Pharmaceutical Sciences Hokkaido University:crest Japan Science And Technology Ag

• Hamada Hirofumi

  Department Of Molecular Biotherapy Research Cancer Center Japanese Foundtion For Cancer Research

• Kato Kazunori

  Department Of Immunology Juntendo University School Of Medicine

• Kogure Kentaro

  Kyoto Pharmaceutical University

• Harashima Hideyoshi

  Graduate School Of Pharmaceutical Sciences Hokkaido University:crest Japan Science And Technology Corporation (jst)

• Nakamura Takashi

  Graduate School Of Engineering Osaka University

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