Noninvasive and persistent transfollicular drug delivery system using a combination of liposomes and iontophoresis
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概要
- 論文の詳細を見る
Iontophoresis is a promising technique for enhancing transdermal administration of charged drugs. However, conventional iontophoresis is not sufficient for effective delivery of large, hydrophilic, or electrically neutral molecules. In this study, we utilized charged liposomes as carriers, focused on a transfollicular route for delivery of the liposomes, and optimized iontophoretic conditions and lipid composition for this method in both in vitro and in vivo conditions. As a result, we identified the optimum condition (lipid composition: DOTAP/EPC/Chol = 2:2:1, current supply: 0.45 mA/cm2, duration: 1 hr) for effective iontophoretic delivery of aqueous solution, which can not be transferred into the skin without charged liposomes. We also examined the pharmacological effects of iontophoresis of liposomes encapsulating insulin (INS-lipo) using a rat model of type I diabetes. Interestingly, iontophoresis of INS-lipo onto a diabetes rat skin resulted in a gradual decrease in blood glucose levels, with levels reaching 20% of initial values at 18 hr after administration. These lower blood glucose levels were maintained for up to 24 hr. Significant amount of insulin were also detected in plasma 18 hr after iontophoresis of INS-lipo. We succeeded in developing a non-invasive and persistent transfollicular drug delivery system that used a combination of liposomes and iontophoresis.
- 2011-01-17
著者
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原島 秀吉
北大院・薬学研究科
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梶本 和昭
北海道大学大学院薬学研究院
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Kogure Kentaro
Dep. Of Biophysical Chemistry Kyoto Pharmaceutical Univ.
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SHIGENOBU Kohki
Faculty of Pharmaceutical Sciences, University of Tokyo
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KANAMURA Kiyoshi
TTI ellebeau Inc.
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Harashima Hideyoshi
Department Of Pharmacokinetics And Pharmaceutics Graduate School Of Pharmaceutical Sciences The Univ
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Kajimoto Kazuaki
Department Of Epidemiology Divisions Of Preventive Medicine
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Harashima Hideyoshi
Fac. Of Pharmaceutical Sciences Hokkaido Univ. Kita-12 Nishi-6 Kita-ku Sapporo 060-0812 Jpn
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Kogure Kentaro
Kyoto Pharmaceutical University
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Kigasawa Kaoru
Graduate School Of Life Sci. Hokkaido Univ.
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Kajimoto Kazuaki
Fac. Of Pharmaceutical Sciences Hokkaido Univ.
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