Quantitative Comparison of Adipocytokine Gene Expression during Adipocyte Maturation in Non-obese and Obese Rats
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概要
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Adipocytokines secreted from adipocytes have been extensively analyzed due to their role as key factors in various complications of obesity, including arterial sclerosis, liver steatosis, insulin resistance, and diabetes. Several in vivo and in vitro studies have suggested that adipocyte maturation is related to fluctuations in adipocytokine secretion. However, the relationship between adipocyte maturation and adipocytokine levels has not been fully elucidated. Therefore, we sought to clarify the link between adipocytokine gene expression and adipocyte maturation through systematic analysis. We quantified mRNA for six adipocytokine genes: adiponectin, resistin, leptin, plasminogen activator inhibitor 1 (PAI-1), heparin-binding epidermal growth factor (EGF)-like growth factor (HB-EGF), and visfatin, in adipose tissue, in primary cultured adipocytes obtained from an obese Zucker rat, and in the preadipocyte cell line 3T3-L1. Moreover, to elucidate the role of adipocytokines in adipocyte maturation, adipocytokine expression levels were analyzed during maturation. Although fluctuations in adipocytokine gene expression were heterogeneous, gene expression was highly similar during maturation of primary cultured adipocytes from obese and non-obese rats, suggesting that the maturation process is independent from processes that lead to obesity. Moreover, the expression patterns of adiponectin, resistin and leptin mRNA in 3T3-L1 cells were highly similar to those in primary cultured adipocytes, indicating that these adipocytokines could be common maturation markers for primary cultured adipocytes obtained from obese and non-obese rats, and for preadipocyte cell lines.
- 公益社団法人 日本薬学会の論文
著者
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梶本 和昭
北海道大学大学院薬学研究院
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TSUCHIYA HIROYUKI
Department of Orthopaedic Surgery, Kanagawa University Graduate School of Medical Sciences
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Kogure Kentaro
Department Of Biophysical Chemistry Kyoto Pharmaceutical University
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Tsuchiya Hiroyuki
Department Of Orthopaedic Surgery Graduate School Of Medical Science Kanazawa University
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Okuno Takashi
Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama
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Kajimoto Kazuaki
Laboratory of Innovative Nanomedicine, Graduate School of Pharmaceutical Sciences, Hokkaido Universi
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Okuno Takashi
Graduate School Of Medicine And Pharmaceutical Sciences University Of Toyama
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Ikeda Yoshito
Department Of Physics Tokyo Institute Of Technology:sony Magnetic Products Inc.
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Hama Susumu
Department of Biophysical Chemistry, Kyoto Pharmaceutical University
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Kajimoto Kazuaki
Department Of Epidemiology Divisions Of Preventive Medicine
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Hama Susumu
Kyoto Pharmaceutical University
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Tsuchiya Hiroyuki
Department of Biophysical Chemistry, Kyoto Pharmaceutical University
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Kajimoto Kazuaki
Laboratory of Innovative Nanomedicine, Graduate School of Pharmaceutical Sciences, Hokkaido University
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Ikeda Yoshito
Department of Biophysical Chemistry, Kyoto Pharmaceutical University
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