Histological examination of frozen autograft treated by liquid nitrogen removed after implantation
スポンサーリンク
概要
- 論文の詳細を見る
Background: Several oncological sterilization methods involving autoclaving, irradiation, or pasteurization have been developed for limb reconstruction of large bone defects following tumor excision. Studies involving histological examinations of these autografts have all found that osteogenesis occurs slowly. We have used frozen autografts treated by liquid nitrogen for limb reconstruction and have achieved excellent results for bone union. To determine if frozen autografts exhibit early bone remodeling, we investigated the repair processes of the frozen bones. Methods: We analyzed frozen autografts treated by liquid nitrogen, retrieved at a mean of 19.1 months (2-75 months) after implantation because of complications or local tumor recurrence. The specimens were obtained from six patients with a mean age of 36.2 years (8-68 years). The six grafts comprised three osteoarticular grafts, two intercalary grafts, and one joint graft. We histologically reviewed the autograft-containing sections for tumor cell necrosis, evidence of cortical repair, the cortical junction, and joint cartilage. Results: Tumor cells were completely eradicated from the frozen bone in all cases. In a specimen retrieved 5 months after implantation, a small area of the bone showed active osteocytes and osteoblasts. In three cases retrieved more than 1 year after implantation, osteocytes and osteoblasts were observed in broad portions of the frozen bones, indicating the onset of osteogenesis in the frozen bone at an early stage. The cortical host-graft junction showed incorporation along with continuity of bone trabeculae. In addition, we were able to fi nd normal chondrocytes on the articular surface. Conclusions: The frozen bone specimens in this study thus showed evidence of newly formed bone and earlier osteogenesis than has been previously reported. Our results suggest that frozen autografts may be considered one of the most useful recycled materials for biological reconstruction. © 2009 The Japanese Orthopaedic Association.
- Springer Verlag (Germany) / 日本整形外科学会 = the Japanese Orthopaedic Associationの論文
- 2009-11-01
著者
-
Kurabayashi Takumi
弘前大学 医学部産科婦人科学
-
Tomita K
Department Of Orthopaedic Surgery Graduate School Of Medical Science Kanazawa University
-
Tomita Katsuro
Department Of Orthopedic Surgery Kanazawa University Graduate School Of Medical Science
-
TSUCHIYA HIROYUKI
Department of Orthopaedic Surgery, Kanagawa University Graduate School of Medical Sciences
-
Tomita Katsuro
Department Of Orthopaedic Surgery Graduate School Of Medical Science Kanazawa University
-
HAYASHI Katsuhiro
Department of Pharmaceutical Sciences, Tohoku University Hospital and Division of Clinical Pharmacy,
-
TANZAWA YOSHIKAZU
Department of Orthopaedic Surgery, Kanazawa University School of Medicine
-
Tsuchiya Hiroyuki
Kanazawa Univ. Kanazawa Jpn
-
Tsuchiya H
Department Of Orthopaedic Surgery Graduate School Of Medical Science Kanazawa University
-
Tsuchiya Hiroyuki
Department Of Orthopaedic Surgery Graduate School Of Medical Science Kanazawa University
-
SHIRAI TOSHIHARU
Department of Orthopaedic Surgery, Graduate School of Medical Science, Kanazawa University
-
Hayashi Katsuhiro
Department Of Orthopaedic Surgery Graduate School Of Medical Science Kanazawa University
-
Tanzawa Yoshikazu
Department Of Orthopaedic Surgery Kanazawa University School Of Medicine
-
Tanzawa Yoshikazu
Department Of Orthopaedic Surgery Ishikawa Central Prefectural Hospital
-
Yo Zen
Department Of Pathology Kanazawa University Hospital
-
Zen Yo
Department Of Pathology Fukui Saiseikai Hospital
-
Shirai Toshiharu
Department Of Orthopaedic Surgery Graduate School Of Medical Science Kanazawa University
-
Tanzawa Yoshikazu
Department Of Orthopaedic Surgery Graduate School Of Medical Science Kanazawa University
-
Tomita Katsuro
Department Of Orthopaedic Surgery Kanazawa University Graduate School Of Medical Science
-
Tsuchiya Hiroyuki
Department Of Orthopaedic Surgery Kanazawa University Hospital
-
Tsuchiya Hiroyuki
Department of Biophysical Chemistry, Kyoto Pharmaceutical University
-
TSUCHIYA Hiroyuki
Department of Orthopaedic Surgery, Kanazawa University Hospital
-
HAYASHI KATSUHIRO
Department of Orthopaedics, Graduate School of Medicine, Kanazawa University
関連論文
- Validation of radiographic response evaluation criteria of preoperative chemotherapy for bone and soft tissue sarcomas : Japanese Orthopaedic Association Committee on Musculoskeletal Tumors Cooperative Study
- Design, Production, and Characterization of Recombinant Neocarzinostatin Apoprotein in Escherichia coli
- Total en bloc spondylectomy for spinal metastases
- Report on the Japanese Orthopaedic Association's 3-year project observing hip fractures at fixed-point hospitals
- Recapping T-saw laminocostotransversoplasty for ventral meningiomas in the thoracic region
- Circumspinal decompression with dekyphosis stabilization for thoracic OPLL
- Finite-element analysis on closing-opening correction osteotomy for angular kyphosis of osteoporotic vertebral fractures
- Invasive features of spinal osteosarcoma obtained from whole-mount sections of total en bloc spondylectomy
- Total en bloc spondylectomy for spinal tumors : improvement of the technique and its associated basic background
- The Transmission of Stress to Grafted Bone Inside a Titanium Mesh Cage Used in Anterior Column Reconstruction After Total Spondylectomy : A Finite-Element Analysis
- Influence on Spinal Cord Blood Flow and Function by Interruption of Bilateral Segmental Arteries at Up to Three Levels : Experimental Study in Dogs
- Influence of Acute Shortening on the Spinal Cord : An Experimental Study
- Letters to the Editor
- Interruption of the Bilateral Segmental Arteries at Several Levels : Influence on Vertebral Blood Flow
- Reconstruction After Total Sacrectomy Using a New Instrumentation Technique : A Biomechanical Comparison
- Biomechanical evaluation of reconstructed lumbosacral spine after total sacrectomy
- Letters
- Mechanical Evaluation of Reconstructed Structures after Total Sacrectomy and Their Improvement
- Anterior lumbar interbody fusion using two standard cylindrical threaded cages, a single mega-cage, or dual nested cages : a biomechanical comparison
- Closing-Opening Wedge Osteotomy to Correct Angular Kyphotic Deformity by a Single Posterior Approach
- Surgical Strategy for Spinal Metastases
- Histological examination of frozen autograft treated by liquid nitrogen removed 6 years after implantation
- Histological examination of autoclaved bone removed 12 years after it was transplanted
- Adamantinoma of the proximal femur : a case report
- Bilateral symmetrical cysts in the upper tibiae in a skeletally mature patient : might they be simple bone cysts?
- Bilateral symmetrical cysts in the upper tibiae in a skeletally mature patient : might they be simple bone cysts?
- Pathological findings of an autograft containing osteosarcoma treated by liquid nitrogen retrieved 2 years after implantation
- Study of the Large-Scale Temperature Structure of the Perseus Cluster with Suzaku
- Suzaku Observation of the Metallicity Distribution in the Elliptical Galaxy NGC 4636
- Adjacent Segment Motion After a Simulated Lumbar Fusion in Different Sagittal Alignments : A Biomechanical Analysis
- Effect of heat shock preconditioning on ROS scavenging activity in rat skeletal muscle after downhill running
- Effect of eccentric exercise on the healing process of injured patellar tendon in rats
- ROS Scavenging Activity and Muscle Damage Prevention in Eccentric Exercise in Rats
- Osteofibrous dysplasia treated with distraction osteogenesis : a report of two cases
- Ankle arthrodesis and tibial lengthening for congenital sensory neuropathy with anhidrosis
- Caffeine-potentiated chemotherapy for metastatic osteosarcoma
- Occlusive dressing for large soft tissue defects following soft tissue tumor excision
- Impact of serum caffeine monitoring on adverse effects and chemotherapeutic responses to caffeine-potentiated chemotherapy for osteosarcoma
- Revision high tibial osteotomy with the Taylor spatial frame for failed opening-wedge high tibial osteotomy
- Ilizarov deformity correction of the lower limbs in Ellis-van Creveld syndrome
- Treatment of lower limb deformities and limb-length discrepancies with the external fixator in Ollier's disease
- Double-level correction with the Taylor Spatial Frame for shepherd's crook deformity in fibrous dysplasia
- Correction and lengthening for deformities of the forearm in multiple cartilaginous exostoses
- Distraction osteogenesis of a fresh fracture site using an external fixator
- Distraction osteogenesis after irradiation in a rabbit model
- Distraction osteogenesis of a previously irradiated femur with malignant lymphoma : a case report
- Correction of juxtaarticular deformities in children using the Ilizarov apparatus
- Successful management of complications from distraction osteogenesis after osteosarcoma resection : a case report
- Bone transport with frozen devitalized bone : an experimental study using rabbits and a clinical application
- Gradual closed correction of equinus contracture using the Ilizarov apparatus
- Osteosarcoma arising from desmoplastic fibroma treated 16 years earlier : a case report
- Intentional marginal excision of osteosarcoma of the proximal fibula to preserve limb function
- Correction of hallux valgus deformity using the center of rotation of angulation method
- Ankle arthrodesis combined with tibial lengthening using the Ilizarov apparatus
- The relationship between distraction length and treatment indices during distraction osteogenesis
- Serum interleukin-6 response after spinal surgery : estimation of surgical magnitude
- Osteoblast lineage properties in giant cell tumors of bone
- Spontaneous elimination of hepatitis C virus RNA in individuals with persistent infection in a hyperendemic area of Japan
- Development of a rapid semi-quantitative immunochromatographic assay for serum hepatocyte growth factor and its usefulness in acute liver failure
- Molecular mechanisms of hereditary persistence of α-fetoprotein (AFP) in two Japanese families : A hepatocyte nuclear factor-1 site mutation leads to induction of the AFP gene expression in adult livers
- Transforming growth factor-β-induced stimulation of formation of collagen fiber network and anti-fibrotic effect of taurine in an in vitro model of hepatic fibrosis
- Enhanced expression of growth factors and imbalance between hepatocyte proliferation and apoptosis in the livers of rats fed a choline-deficient, L-amino acid-defined diet
- Bridging Use of Plasma Exchange and Continuous Hemodiafiltration before Living Donor Liver Transplantation in Fulminant Wilson's Disease
- A case of acute hepatitis A with marked hemophagocytosis in bone marrow
- Three-dimensional morphological analysis of the proximal femoral canal, using computer-aided design system, in Japanese patients with osteoarthrosis of the hip
- Induction of tumor necrosis factor-α in Schwann cells after gradual elongation of rat sciatic nerve
- Effects of liquid nitrogen treatment on the proliferation of osteosarcoma and the biomechanical properties of normal bone
- Histological and radiological analysis of autoclaved bone 2 years after extirpation
- Conversion disorder : four case reports concerning motor disorder symptoms
- Changes of the soluble fibrin monomer complex level during the perioperative period of hip replacement surgery
- Distraction osteogenesis for treatment of bone loss in the lower extremity
- Proximal interphalangeal joint silicone arthroplasty for posttraumatic arthritis
- A New Method to Evaluate the Unfolding Activity of Chaperone Unit ClpA Based on Fe–S Cluster Disruption
- Exogenously expressed granulocyte colony-stimulating factor (G-CSF) receptor on K562 cells can transduce G-CSF-triggered growth and differentiation signals
- The association of neurofibromatosis 1 and spinal deformity with primary hyperparathyroidism and osteomalacia : might melatonin have a role?
- Newborn hearing screening in a single private Japanese obstetric hospital
- Regeneration of Lymphatic Vessels and Lipometabolism in an Experimental Rat Model of Extensive Dissection of the Abdominal Periaortic Lymphatic Vessels and Lymph Nodes
- Fatal cytomegalovirus myocarditis in a seronegative ALL patient
- Correction of tibial deformity in adults
- Three-Dimensional Analysis of Thoracic Apical Sagittal Alignment in Adolescent Idiopathic Scoliosis
- Surgical management of aggressive vertebral hemangiomas causing spinal cord compression : long-term clinical follow-up of five cases
- Total en bloc spondylectomy and a greater omentum pedicle flap for a large bone and soft tissue defect : solitary lumbar metastasis from renal cell carcinoma
- Surgical site infection in spinal metastasis: risk factors and countermeasures.
- Effects on Spinal Cord Blood Flow and Neurologic Function Secondary to Interruption of Bilateral Segmental Arteries Which Supply the Artery of Adamkiewicz : An Experimental Study Using a Dog Model
- Influence of ligation of the internal iliac veins on the venous plexuses around the sacrum
- A case of hepatocellular carcinoma associated with autoimmune hepatitis
- Pedicle frozen autograft reconstruction in malignant bone tumors
- Histological examination of frozen autograft treated by liquid nitrogen removed after implantation
- Quantitative Comparison of Adipocytokine Gene Expression during Adipocyte Maturation in Non-obese and Obese Rats
- Total En Bloc Spondylectomy of the Lower Lumbar Spine : A Surgical Techniques of Combined Posterior-Anterior Approach
- Local application of olprinone for promotion of peripheral nerve regeneration
- Effect of near-infrared light-emitting diodes on nerve regeneration
- An Analysis of Decision Making and Treatment in Thoracolumbar Metastases
- Extraskeletal myxoid chondrosarcoma with multiple skeletal metastases
- Patient-Specific Finite Element Analyses Detect Significant Mechanical Therapeutic Effects on Osteoporotic Vertebrae During a Three-Year Treatment
- High-grade undifferentiated pleomorphic sarcoma of pelvis treated with curettage and bone graft after complete remission following caffeine-potentiated chemotherapy
- Potentiation of the antitumor effect of calcium phosphate cement containing anticancer drug and caffeine on rat osteosarcoma
- The route of metastatic vertebral tumors extending to the adjacent vertebral body : a histological study
- Mechanical Therapeutic Effects in Osteoporotic L1-Vertebrae Evaluated by Nonlinear Patient-specific Finite Element Analysis