Direct Monitoring of Interaction between Escherichia coli Proteins, MinC and Monomeric FtsZ, in Solution

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概要

• 論文の詳細を見る

• MinC plays an important role in regulation of the cell division site in Escherichia coli. Previous studies using sedimentation and electron microscopic methods suggested that MinC interacts with the FtsZ polymer and inhibits further FtsZ polymerization. However, it is difficult to clarify details regarding specific molecular interactions by such static analytic methods. In this study, a fluorescence resonance energy transfer (FRET) method was developed to directly observe the interaction between Cy3-labeled MinC and Cy5-labeled FtsZ in solution. FRET analysis indicated that MinC interacts with monomeric rather than polymeric FtsZ in solution. This suggests that interactions between monomeric FtsZ and MinC are important for controlling of FtsZ polymerization by MinC.

• 公益社団法人　日本薬学会の論文

著者

• Funasaki Noriaki

  Department of Physical Chemsitry, Kyoto Pharmaceutical University

• Okuno T

  Graduate School Of Medicine And Pharmaceutical Sciences Univ. Of Toyama

• Kogure Kentaro

  Department Of Biophysical Chemistry Kyoto Pharmaceutical University

• Okuno Takashi

  Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama

• Okuno Takashi

  Graduate School Of Medicine And Pharmaceutical Sciences University Of Toyama

• Funasaki Noriaki

  Department Of Physical Chemistry 21st Century Coe Program Kyoto Pharmaceutical University

• Ogoh Machiko

  Department of Biophysical Chemistry, Kyoto Pharmaceutical University

• Tanina Hiromitsu

  Department of Biophysical Chemistry, Kyoto Pharmaceutical University

• Funasaki Noriaki

  Department of Biophysical Chemistry, Kyoto Pharmaceutical University

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