Cell penetrating peptide-mediated systemic siRNA delivery to the liver
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概要
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The cell-penetrating peptide (CPP) is one of the most attractive tools for efficiently delivering biomolecules to a target organelle. Here, we describe the use of octaarginine (R8)-modified lipid nanoparticles for the efficient and targeted in vivo delivery of siRNA to the liver. In this study, SR-BI (a scavenger receptor class B, member 1) was targeted by this nanoparticle. Our results demonstrate that R8-modified lipid nanoparticles can be used for the efficient and targeted delivery of liver siRNA to induce the specific knock-down of an endogenous gene with minimum liver toxicity and immune response, and that this CPP based technology holds considerable promise for further in vivo biological applications of siRNA.
- 2011-10-31
著者
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原島 秀吉
北大院・薬学研究科
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梶本 和昭
北海道大学大学院薬学研究院
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Akita Hidetaka
Lab. For Molecular Design Of Pharmaceutics Fac. Of Pharmaceutical Sciences Hokkaido Univ.
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Shim Chang
Faculty Of Pharmaceutical Sciences University Of Tokyo : (present Address) College Of Pharmacy Seoul
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SHIGENOBU Kohki
Faculty of Pharmaceutical Sciences, University of Tokyo
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Harashima Hideyoshi
Department Of Pharmacokinetics And Pharmaceutics Graduate School Of Pharmaceutical Sciences The Univ
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Akita Hidetaka
Graduate School Of Pharmaceutical Sciences Hokkaido University
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Hayashi Yasuhiro
Department Of Surgical Oncology Graduate School Of Medicine Hokkaido University
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Kajimoto Kazuaki
Department Of Epidemiology Divisions Of Preventive Medicine
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Kajimoto Kazuaki
Fac. Of Pharmaceutical Sciences Hokkaido Univ.
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