Correction of Frameshift Mutations with Tailed Duplex DNAs
スポンサーリンク
概要
- 論文の詳細を見る
Tailed duplex (TD) DNAs, prepared by annealing an oligonucleotide to a several-hundred-base single-stranded (ss) DNA fragment, correct a base-substitution mutation with high efficiency. In the present study, the abilities of TD fragments to correct single-base insertion and deletion mutations were examined, using hygromycin-resistance and enhanced green fluorescent protein fusion (Hyg-EGFP) genes inactivated by +G and -C frameshift mutations. The 5'-TD and 3'-TD DNA fragments were co-transfected with plasmid DNA containing the inactivated Hyg-EGFP gene into CHO-K1 cells, and the gene correction efficiencies were determined by introducing the plasmid DNA recovered from the transfected cells into Escherichia coli cells. In contrast to their efficiencies for the substitution mutation, the gene correction abilities of the TD fragments were relatively low. The correction efficiencies by the TD fragments were apparently higher than that by a ss DNA fragment, one of the DNA fragments employed for gene correction. These results suggest that the TD fragments have the potential to correct frameshift mutations, although further improvement is required.
- Pharmaceutical Society of Japanの論文
著者
-
Shim Chang
Faculty Of Pharmaceutical Sciences University Of Tokyo : (present Address) College Of Pharmacy Seoul
-
Tsuchiya Hiroyuki
Division Of Molecular And Genetic Medicine Department Of Genetic Medicine And Regenerative Therapeut
-
Kamiya Hiroyuki
Faculty Of Pharmaceutical Sciences Hokkaido University
-
Harashima Hideyoshi
Faculty Of Pharmaceutical Science The University Of Tokushima
-
Tsuchiya Hiroyuki
Division Of Molecular And Genetic Medicine Department Of Genetic Medicine And Regenerative Therapeut
-
Tsuchiya Hiroyuki
Division Of Molecular And Genetic Medicine Department Of Genetic Medicine And Regenerative Therapeut
-
Morita Yukiko
Faculty of Pharmaceutical Sciences, Hokkaido University
-
Tsuchiya Hiroyuki
Faculty of Pharmaceutical Sciences, Hokkaido University
関連論文
- Suppression of mutagenesis by 8-hydroxy-2'-deoxyguanosine 5'-triphosphate (7,8-dihydro-8-oxo-2'-deoxyguanosine 5'-triphosphate) by human MTH1, MTH2, and NUDT5
- Base excision repair enzyme endonuclease III suppresses mutagenesis caused by 8-hydroxy-dGTP
- UvrA and UvrB enhance mutations induced by oxidized deoxyribonucleotides
- Mutations Induced by Oxidized DNA Precursors and Their Prevention by Nucleotide Pool Sanitization Enzymes
- Correlation between the Phosphohydrolase Activity of the Escherichia coli Orf135 (NudG) Protein and Mutation Suppression
- Comparison between a Multifunctional Envelope-Type Nano Device and Lipoplex for Delivery to the Liver
- Ligand-based targeted delivery of a peptide modified nanocarrier to endothelial cells in adipose tissue
- Involvement of thioredoxin-binding protein 2 in the antitumor activity of CD437
- Ornithine and Tryptophan Analogs as Efficient Polycations for Short Interference RNA Delivery to Tumor Cells
- Genetic analysis of expression profile involved in retinoid metabolism in non-alcoholic fatty liver disease
- MITO-Porter : a liposome-based carrier system for delivery of macromolecules into mitochondria via membrane fusion
- In Vitro Efficacy of a Sterically Stabilized Immunoliposomes Targeted to Membrane Type 1 Matrix Metalloproteinase (MT1-MMP)(Biopharmacy)
- Recognition of Nucleotide Analogs Containing the 7,8-Dihydro-8-oxo Structure by the Human MTH1 Protein
- Kinetic Analysis of the Positive Inotropic Action (PIA) of Ouabain in Isolated Perfused Rabbit Heart. Slow Onset of PIA and Slow Binding to Na^+, K^+-Adenosine Triphosphatase
- PREDICTION OF SERUM CONCENTRATION TIME COURSE OF QUINIDINE IN HUMAN USING A PHYSIOLOGICALLY BASED PHARMACOKINETIC MODEL DEVELOPED FROM THE RAT
- PREDICTION OF THE PLASMA CONCENTRATION TIME COURSES OF VARIOUS DRUGS IN HUMANS BASED ON DATA FROM RATS
- Hepatic differentiation of human bone marrow-derived UE7T-13 cells : Effects of cytokines and CCN family gene expression
- The liver as a target organ of retinoids
- DNA Microarray Analysis of Whole Blood Cells and Insulin-Sensitive Tissues Reveals the Usefulness of Blood RNA Profiling as a Source of Markers for Predicting Type 2 Diabetes
- DNA Microarray Analysis of Type 2 Diabetes-Related Genes Co-regulated between White Blood Cells and Livers of Diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) Rats
- NUDT5 hydrolyzes oxidized deoxyribonucleoside diphosphates with broad substrate specificity
- Mutagenic effects of 8-hydroxy-dGTP in live mammalian cells
- Induction of Substitution and Deletion Mutations by 2-Hydroxyadenine during Replication in a HeLa Extract
- Incorporation of 8-hydroxyguanosine (8-oxo-7,8-dihydroguanosine) 5'-triphosphate by bacterial and human RNA polymerases
- Genome Wide Expression Analysis of White Blood Cells and Liver of Pre-diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) Rats Using a cDNA Microarray
- Cytotoxic Effect of Drosophila Deoxynucleoside Kinase Gene on Replicating Plasmid in HeLa Cells
- Induction of Various Mutations during PCRs with Manganese and 8-Hydroxy-dGTP
- PK-PD modeling of 1-(3-C-ethynyl-β-D-ribo-pentofuranosyl)cytosine and the enhanced antitumor effect of its phospholipid derivatives in long-circulating liposomes
- Correction of Frameshift Mutations with Single-Stranded and Double-Stranded DNA Fragments Prepared from Phagemid/Plasmid DNAs(Miscellaneous)
- Mutagenicity of 5-Formylcytosine, an Oxidation Product of 5-Methylcytosine, in DNA in Mammalian Cells
- Effects of Target Sequence and Sense versus Anti-sense Strands on Gene Correction with Single-stranded DNA Fragments
- In Vivo Studies on the Role of Complement in the Clearance of Liposomes in Rats and Guinea Pigs
- Effects of non-B DNA sequences on transgene expression
- Enhanced antigen presentation and CTL activity by transduction of mature rather than immature dendritic cells with octaarginine-modified liposomes
- Cell cycle dependent transcription, a determinant factor of heterogeneity in cationic lipid-mediated transgene expression.
- Effects of 8-hydroxy-GTP and 2-hydroxy-ATP on in vitro transcription.
- Effect of Polyethyleneglycol Spacer on the Binding Properties of Nuclear Localization Signal-Modified Liposomes to Isolated Nucleus
- Hepatocyte growth factor mobilizes and recruits hematopoietic progenitor cells into liver through a stem cell factor-mediated mechanism
- Kinetic Analysis of the Interaction between Liposomes and the Complement System in Rat Serum : Re-evaluation of Size-Dependency
- Complement Dependent and Independent Liposome Uptake by Peritoneal Macrophages : Cholesterol Content Dependency
- Effects of Repeated Clarithromycin Administration on the Pharmacokinetic Properties of Pindolol in Rats
- KINETIC ANALYSIS OF THE UPTAKE PROCESS OF LIPOSOMES BY RES
- Novel lipidated sorbitol-based molecular transporters for non-viral gene delivery
- Efficient Short Interference RNA Delivery to Tumor Cells Using a Combination of Octaarginine, GALA and Tumor-Specific, Cleavable Polyethylene Glycol System
- Comparison of Incorporation and Extension of Nucleotides in vitro opposite 8-Hydroxyguanine (7,8-Dihydro-8-oxoguanine) in Hot Spots of the c-Ha-ras Gene
- Mitochondrial drug delivery and mitochondrial disease therapy--an approach to liposome-based delivery targeted to mitochondria.
- A20 Silencing by Lipid Envelope-Type Nanoparticles Enhances the Efficiency of Lipopolysaccharide-Activated Dendritic Cells
- Correction of frameshift mutations with tailed duplex DNAs
- Cell penetrating peptide-mediated systemic siRNA delivery to the liver
- Iron state in association with retinoid metabolism in non-alcoholic fatty liver disease
- A Close Association of Abnormal Iron Metabolism with Steatosis in the Mice Fed a Choline-Deficient Diet
- PREDICTION OF RENAL TUBULAR SECRETION OF TETRAETHYLAMMONIUM BROMIDE BY USE OF ENDOGENOUS N^1-METHYLNICOTINAMIDE IN THE RAT
- Unexpectedly Weak Impacts of Decreased p53 and Retinoblastoma Protein Levels on Mutagenesis by 8-Oxo-7, 8-dihydroguanine (8-Hydroxyguanine)
- c-Jun N-terminal kinase activation by oxidative stress suppresses retinoid signaling through proteasomal degradation of retinoic acid receptor α protein in hepatic cells
- Disrupted plasma membrane localization of equilibrative nucleoside transporter 2 in the chemoresistance of human pancreatic cells to gemcitabine (dFdCyd)
- Effects of insulator cHS4 on transgene expression from plasmid DNA in a positive feedback system(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- A DNA Microarray-based Analysis of the Host Response to a Nonviral Gene Carrier: A Strategy for Improving the Immune Response
- 2D1636 Ferredoxinをモデル基質とする大腸菌シャペロンClpAの新規アンフォールディング活性評価法の確立(蛋白質_構造機能相関1,第49回日本生物物理学会年会)
- Intracellular stability of 2'-OMe-4'-thioribonucleoside modified siRNA leads to long-term RNAi effect
- Correction of Frameshift Mutations with Tailed Duplex DNAs
- Effects of Endogenous Proteins and microRNA Target Sequence in a Positive Feedback System
- Effect of the Anchor in Polyethylene Glycol-Lipids on the Transfection Activity of PEGylated Cationic Liposomes Encapsulating DNA
- Efficient Intradermal Delivery of Superoxide Dismutase Using a Combination of Liposomes and Iontophoresis for Protection against UV-Induced Skin Damage
- Reduced Plasma Glucose by Asparagine Synthetase Knockdown in the Mouse Liver
- Production of small nano-sized particles by complex formation between polycations and linearized plasmid DNA at a low pH(METHODS)