Effects of Endogenous Proteins and microRNA Target Sequence in a Positive Feedback System
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概要
- 論文の詳細を見る
A positive feedback system, using GAL4-vp16 (a fusion protein of yeast GAL4 and herpes simplex virus vp16) as an activator and firefly luciferase as a reporter, maintained luciferase expression for 7 d in mice. However, the luciferase expression decreased after 7 d, and this phenomenon could be caused by immunoreactions against these exogenous proteins. This hypothesis was examined by the following three strategies, designed to avoid the putative immunoreactions: (i) use of the endogenous secreted alkaline phosphatase (SEAP) protein as a reporter, (ii) replacement of vp16 with endogenous transcription factors, and (iii) insertion of the target sequence of microRNA expressed in cells of hematopoietic origin, to suppress GAL4-vp16 expression in antigen-presenting cells. The results obtained in this study suggested that silencing would be induced by mechanism(s) besides immunoreactions against reporter and activator proteins.
- 公益社団法人 日本薬学会の論文
著者
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Harashima Hideyoshi
Faculty of Pharmaceutical Sciences, Hokkaido University
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Kamiya Hiroyuki
Faculty Of Pharmaceutical Sciences Hokkaido University
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Harashima Hideyoshi
Faculty Of Pharmaceutical Science The University Of Tokushima
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Kanda Genki
Faculty of Pharmaceutical Sciences, Hokkaido University
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Togashi Ryohei
Faculty of Pharmaceutical Sciences, Hokkaido University
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