Effects of Target Sequence and Sense versus Anti-sense Strands on Gene Correction with Single-stranded DNA Fragments
スポンサーリンク
概要
- 論文の詳細を見る
The correction of an inactivated hygromycin resistance and enhanced green fluorescent protein (Hyg–EGFP) fusion gene by a several hundred-base single-stranded (ss) DNA fragment has been reported. In this study, the effectiveness of this type of gene correction was examined for various positions in the rpsL gene. Sense and anti-sense ssDNA fragments were prepared, and the gene correction efficiencies were determined by co-introduction of the target plasmid containing the gene with the ssDNA fragments. The gene correction efficiency varied (0.8–9.3%), depending on target positions and sense/anti-sense strands. Sense ssDNA fragments corrected the target gene with equal or higher efficiencies as compared to their anti-sense counterparts. The target positions corrected with high efficiency by the sense fragments also tended to be corrected efficiently by the anti-sense fragments. These results suggest that the sense ssDNA fragments are useful for the correction of mutated genes. The variation in the correction efficiency may depend on the sequence of the target position in double-stranded DNA.
- The Japanese Biochemical Societyの論文
- 2008-10-01
著者
-
Kamiya Hiroyuki
Faculty of Pharmaceutical Sciences, Hokkaido University
-
Harashima Hideyoshi
Faculty of Pharmaceutical Sciences, Hokkaido University
-
UCHIYAMA Masayuki
Faculty of Pharmaceutical Sciences, Hokkaido University
-
NAKATSU Yoshimichi
Faculty of Medical Sciences, Kyushu University
-
TSUZUKI Teruhisa
Faculty of Medical Sciences, Kyushu University
-
Kamiya Hiroyuki
Faculty Of Pharmaceutical Sciences Hokkaido University
-
Harashima Hideyoshi
Faculty Of Pharmaceutical Science The University Of Tokushima
関連論文
- Transgene expression efficiency from plasmid DNA delivered as a complex with histone H3
- Suppression of mutagenesis by 8-hydroxy-2'-deoxyguanosine 5'-triphosphate (7,8-dihydro-8-oxo-2'-deoxyguanosine 5'-triphosphate) by human MTH1, MTH2, and NUDT5
- UvrA and UvrB enhance mutations induced by oxidized deoxyribonucleotides
- Mutations Induced by Oxidized DNA Precursors and Their Prevention by Nucleotide Pool Sanitization Enzymes
- Correlation between the Phosphohydrolase Activity of the Escherichia coli Orf135 (NudG) Protein and Mutation Suppression
- Effects of Overexpression and Antisense RNA Expression of Orf17, a MutT-Type Enzyme
- Ornithine and Tryptophan Analogs as Efficient Polycations for Short Interference RNA Delivery to Tumor Cells
- MITO-Porter : a liposome-based carrier system for delivery of macromolecules into mitochondria via membrane fusion
- Recognition of Nucleotide Analogs Containing the 7,8-Dihydro-8-oxo Structure by the Human MTH1 Protein
- Kinetic Analysis of the Positive Inotropic Action (PIA) of Ouabain in Isolated Perfused Rabbit Heart. Slow Onset of PIA and Slow Binding to Na^+, K^+-Adenosine Triphosphatase
- PREDICTION OF SERUM CONCENTRATION TIME COURSE OF QUINIDINE IN HUMAN USING A PHYSIOLOGICALLY BASED PHARMACOKINETIC MODEL DEVELOPED FROM THE RAT
- PREDICTION OF THE PLASMA CONCENTRATION TIME COURSES OF VARIOUS DRUGS IN HUMANS BASED ON DATA FROM RATS
- New NTP analogs: the synthesis of 4'-thioUTP and 4'-thioCTP and their utility for SELEX
- DNA Microarray Analysis of Type 2 Diabetes-Related Genes Co-regulated between White Blood Cells and Livers of Diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) Rats
- Mutagenic effects of 8-hydroxy-dGTP in live mammalian cells
- Induction of Substitution and Deletion Mutations by 2-Hydroxyadenine during Replication in a HeLa Extract
- Incorporation of 8-hydroxyguanosine (8-oxo-7,8-dihydroguanosine) 5'-triphosphate by bacterial and human RNA polymerases
- Cytotoxic Effect of Drosophila Deoxynucleoside Kinase Gene on Replicating Plasmid in HeLa Cells
- Induction of Various Mutations during PCRs with Manganese and 8-Hydroxy-dGTP
- PK-PD modeling of 1-(3-C-ethynyl-β-D-ribo-pentofuranosyl)cytosine and the enhanced antitumor effect of its phospholipid derivatives in long-circulating liposomes
- Correction of Frameshift Mutations with Single-Stranded and Double-Stranded DNA Fragments Prepared from Phagemid/Plasmid DNAs(Miscellaneous)
- RNA interference induced by siRNAs modified with 4'-thioribonucleosides in cultured mammalian cells
- Effects of Target Sequence and Sense versus Anti-sense Strands on Gene Correction with Single-stranded DNA Fragments
- In Vivo Studies on the Role of Complement in the Clearance of Liposomes in Rats and Guinea Pigs
- Effects of non-B DNA sequences on transgene expression
- Cell cycle dependent transcription, a determinant factor of heterogeneity in cationic lipid-mediated transgene expression.
- Effects of 8-hydroxy-GTP and 2-hydroxy-ATP on in vitro transcription.
- Effect of Polyethyleneglycol Spacer on the Binding Properties of Nuclear Localization Signal-Modified Liposomes to Isolated Nucleus
- Kinetic Analysis of the Interaction between Liposomes and the Complement System in Rat Serum : Re-evaluation of Size-Dependency
- Complement Dependent and Independent Liposome Uptake by Peritoneal Macrophages : Cholesterol Content Dependency
- Effects of Repeated Clarithromycin Administration on the Pharmacokinetic Properties of Pindolol in Rats
- KINETIC ANALYSIS OF THE UPTAKE PROCESS OF LIPOSOMES BY RES
- Comparison of Incorporation and Extension of Nucleotides in vitro opposite 8-Hydroxyguanine (7,8-Dihydro-8-oxoguanine) in Hot Spots of the c-Ha-ras Gene
- Mitochondrial drug delivery and mitochondrial disease therapy--an approach to liposome-based delivery targeted to mitochondria.
- A20 Silencing by Lipid Envelope-Type Nanoparticles Enhances the Efficiency of Lipopolysaccharide-Activated Dendritic Cells
- Unexpectedly Weak Impacts of Decreased p53 and Retinoblastoma Protein Levels on Mutagenesis by 8-Oxo-7, 8-dihydroguanine (8-Hydroxyguanine)
- Effects of insulator cHS4 on transgene expression from plasmid DNA in a positive feedback system(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Mitochondrial delivery of mastoparan with transferrin liposomes equipped with a pH-sensitive fusogenic peptide for selective cancer therapy.
- Correction of Frameshift Mutations with Tailed Duplex DNAs
- Effects of Endogenous Proteins and microRNA Target Sequence in a Positive Feedback System
- Effect of the Anchor in Polyethylene Glycol-Lipids on the Transfection Activity of PEGylated Cationic Liposomes Encapsulating DNA
- Efficient Intradermal Delivery of Superoxide Dismutase Using a Combination of Liposomes and Iontophoresis for Protection against UV-Induced Skin Damage
- Reduced Plasma Glucose by Asparagine Synthetase Knockdown in the Mouse Liver
- Production of small nano-sized particles by complex formation between polycations and linearized plasmid DNA at a low pH(METHODS)