Transgene expression efficiency from plasmid DNA delivered as a complex with histone H3
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概要
- 論文の詳細を見る
The intranuclear disposition of plasmid DNA is extremely important for transgene expression. Exogenous histones have been used as carriers of plasmid DNA in histone-mediated gene delivery. In this study, the effects of exogenous histone H3 complexed with plasmid DNA on transgene expression efficiency were examined. The plasmid-histone complexes in various ratios were transfected into HeLa cells by osmotic pressure. Histone H3 suppressed transgene expression in the nucleus in a dose-dependent manner. Our results suggest that the histone-mediated gene delivery is unlikely to be useful, from the viewpoint of the intranuclear disposition.
- 2010-06-15
著者
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Kamiya Hiroyuki
Faculty of Pharmaceutical Sciences, Hokkaido University
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Kiwada Hiroshi
徳島大学 ヘルスバイオサイエンス研究部薬物動態制御学
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Kiwada Hiroshi
Faculty Of Pharmaceutifal Sciences The University Of Tokushima
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原島 秀吉
北大院・薬学研究科
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Kamada Haruhiko
医薬基盤研究所 創薬プロテオミクスプロジェクト
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Kiwada Hiroshi
Department Of Pharmacokinetics And Biopharmaceutics Faculty Of Pharmaceutical Sciences The Universit
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Yamada Yuma
Lab. For Molecular Design Of Pharmaceutics Fac. Of Pharmaceutical Sciences Hokkaido Univ.
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Yamada Yuma
Laboratory For Molecular Design Of Pharmaceutics Faculty Of Pharmaceutical Sciences Hokkaido Univers
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Kamiya Hiroyuki
Lab. For Molecular Design Of Pharmaceutics Fac. Of Pharmaceutical Sciences Hokkaido Univ.
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SHIGENOBU Kohki
Faculty of Pharmaceutical Sciences, University of Tokyo
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Kasai Hiroshi
Inst. Of Industrial Ecological Sciences Univ. Of Occupational And Environmental Health 1-1 Iseigaoka
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Harashima Hideyoshi
Department Of Pharmacokinetics And Pharmaceutics Graduate School Of Pharmaceutical Sciences The Univ
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Kamiya Hiroyuki
産業医科大学産業生態科学研究所
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Murata-kamiya Naoko
Inst. Indust. Ecolog. Sci. Univ. Occup. Environ. Hith.
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Kamiya Hiroyuki
Faculty Of Pharmaceutical Sciences Hokkaido University:crest Japan Science And Technology
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山田 勇磨
北海道大学大学院薬学研究科薬剤分子設計学分野
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