Lactoferrin Inhibits Platelet Production from Human Megakaryocytes in Vitro(Molecular and Cell Biology)
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概要
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The mechanism of megakaryopoiesis, proplatelet formation (PPF) and platelet (PLT) production is not fully elucidated. Lactoferrin (LF) has been reported to have many biological functions including cell proliferation and differentiation, and the LF receptor is present on megakaryocytic cells. In the present study, we examined the effect of human LF (hLF) on PLT production from primary megakaryocytes (MKs). At first, we developed a PLT production system derived from human CD34^+ cells by thrombopoietin (TPO) stimulation. Because the number of proplatelets, PLTs and CD41^+ MKs was remarkebly increased after day 5, we employed the TPO-induced CD34^+ cells on day 5. Then, the effect of hLF on PLT production from human primary MKs was examined. In the range of 3-30 μg/ml, hLF significantly inhibited PLT production up to about 60%. However, it did not significantly change the intensity of CD41 expression in MKs and the ploidy of MKs. In addition, it did not inhibit MK progenitors. These results suggest that LF directly inhibits PLT production from matured MKs, but does not inhibit megakaryopoiesis, including proliferation/maturation processes.
- 2008-04-01
著者
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Ishida Tatsuhiro
Department of Pharmacokinetics and Biopharmaceutics, Subdivision of Biopharmaceutical Sciences, Inst
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Kiwada Hiroshi
Department of Pharmacokinetics and Biopharmaceutics, Subdivision of Biopharmaceutical Sciences, Inst
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Kiwada Hiroshi
徳島大学 ヘルスバイオサイエンス研究部薬物動態制御学
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Kiwada Hiroshi
Department Of Pharmacokinetics And Biopharmaceutics Subdivision Of Biopharmaceutical Sciences Instit
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Kiwada Hiroshi
Faculty Of Pharmaceutifal Sciences The University Of Tokushima
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MATSUMURA-TAKEDA Kuniko
Department of Pharmacokinetics and Biopharmaceutics, Subdivision of Biopharmaceutical Sciences, Inst
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SOGO Shinji
First Institute of New Drug Discovery, Otsuka Pharmaceutical Co., Ltd.
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ISAKARI Yoshimasa
Department of Pharmacokinetics and Biopharmaceutics, Subdivision of Biopharmaceutical Sciences, Inst
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TAKI Takao
Institute of Biomedical Innovation, Otsuka Pharmaceutical Co., Ltd.
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SUDO Toshiki
First Institute of New Drug Discovery, Otsuka Pharmaceutical Co., Ltd.
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Higashisaka Kazuma
Dep. Of Toxicology And Safety Sci. Graduate School Of Pharmaceutical Sciences Osaka Univ.
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Kamada Haruhiko
医薬基盤研究所 創薬プロテオミクスプロジェクト
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Kamada Haruhiko
Laboratory Of Pharmaceutical Proteomics (lpp) National Institute Of Biomedical Innovation (nibio)
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Kiwada Hiroshi
Department Of Pharmacokinetics And Biopharmaceutics Faculty Of Pharmaceutical Sciences The Universit
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Kamiya Hiroyuki
Lab. For Molecular Design Of Pharmaceutics Fac. Of Pharmaceutical Sciences Hokkaido Univ.
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Sudo Toshiki
First Institute Of New Drug Discovery Otsuka Pharmaceutical Co. Ltd.
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Sogo Shinji
Molecular Medical Science Institute Otsuka Pharmaceutical Co. Ltd.
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Kamada H
Yamagata Technopolis Foundation Yamagata Jpn
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Kamada Hitoshi
Institute For Life Support Technology Yamagata Promotional Organization For Industrial Technology
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Taki T
Molecular Medical Science Institute Otsuka Pharmaceutical Co. Ltd.
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Isakari Yoshimasa
Department Of Pharmacokinetics And Biopharmaceutics Subdivision Of Biopharmaceutical Sciences Instit
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Matsumura-takeda Kuniko
Department Of Pharmacokinetics And Biopharmaceutics Subdivision Of Biopharmaceutical Sciences Instit
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Kamada H
Institute For Life Support Technology Yamagata Promotional Organization For Industrial Technology
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Kiwada H
Faculty Of Pharmaceutifal Sciences The University Of Tokushima
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Kiwada Hiroshi
Department Of Pharmacokinetics And Biopharmaceutics Subdivision Of Biopharmaceutical Science Institu
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Taki Takao
Institute Of Biomedical Innovation Otsuka Pharmaceutical Co. Ltd.
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Ishida Tatsuhiro
Dep. Of Pharmacokinetics And Biopharmaceutics Subdivision Of Biopharmaceutical Sciences Inst. Of Hea
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Ishida Tatsuhiro
Department Of Pharmacokinetics And Biopharmaceutics Subdivision Of Biopharmaceutical Science Institu
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Ishida Tatsuhiro
Department Of Pharmacokinetics And Biopharmaceutics Institute Of Health Biosciences The University O
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