Non-linear pharmacodynamics in a non-viral gene delivery system: Positive non-linear relationship between dose and transfection efficiency
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概要
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http://www.elsevier.com/wps/find/journaldescription.cws_home/502690/descriptionA remarkable non-linearity was found between dose and transfection activities of non-viral gene delivery systems, such as a Lipofectamine/DNA complex and an octaarginine-modified multifunctional envelope-type nano device (R8-MEND). We measured the nuclear delivery of pDNA to distinguish the non-linearity in intracellular pharmacokinetics or pharmacodynamics after transfection with R8-MEND at different doses. A remarkable positive non-linearity was found in the pharmacodynamics when the dose was increased. Even dummy pDNA enhanced the efficiency of transcription and/or translation per pDNA in the nucleus, but empty liposomes did not. These results suggest the importance of controlled pharmacodynamics as well as the importance of intracellular pharmacokinetics for the rational design of non-viral gene delivery systems.
- Elsevier B.Vの論文
著者
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Kogure Kentaro
Dep. Of Biophysical Chemistry Kyoto Pharmaceutical Univ.
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Akita Hidetaka
Lab. For Molecular Design Of Pharmaceutics Fac. Of Pharmaceutical Sciences Hokkaido Univ.
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Harashima Hideyoshi
Department Of Pharmacokinetics And Pharmaceutics Graduate School Of Pharmaceutical Sciences The Univ
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Akita Hidetaka
Graduate School Of Pharmaceutical Sciences Hokkaido University
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Moriguchi Rumiko
Graduate School Of Pharmaceutical Sciences Hokkaido University:crest Japan Science And Technology Ag
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Kogure Kentaro
Kyoto Pharmaceutical University
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