Effects of Incorporation of Various Amphiphiles into Recipient Liposome Membraneson Inter-Membrane Protein Transfer

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概要

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• To obtain informaiton about the factors governing spontaneous inter-membrane protein transfer, we examined the effects of incorporation of various amphiphilic compounds in dimyristoylphosphatidylcholine (DMPC) liposomes on protein transfer from influenza virus-infected cells to the liposomes, and analyzed the physical properties of these liposome membranes. The incorporation of amphiphilic compounds, negatively charged divetylphosphate (DCP), dipalmitoylphosphatidylserine (DPPS) or positively charged dimethyldipalmitolammonium (DMDPA), into DMPC liposomal membranes enhanced protein transfer. The liposomes containing DCP, DPPS or DMDPA were unaffected by osmotic shock caused by external addition of glucose, suggeting a decrease in lipid packing in the liposomal membranes. Furthermore, calorimetric study of these liposomes showed that a phase separation occurred partially in the liposomal membranes. Accordingly, the membranes of DMPC liposomes containing DCP, DPPS and DMDPA should be distorted due to the coexistence of two phases, gel and liquid crystalline, in the membranes. Consequenctly, the membrane distortion could be responsible for the enhancement effects of the amphiphiles on the inter-membrane protein transfer from influenza virus-infected cells to the liposomes.

• 1999-08-15

著者

• Hayashi Kyoko

  Faculty Of Medicine Toyama Medical And Pharmaceutical University

• Kogure Kentaro

  Dep. Of Biophysical Chemistry Kyoto Pharmaceutical Univ.

• Kogure K

  Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University:(present Address)fac

• Kogure Kentaro

  Faculty Of Pharmaceutical Sciences The University Of Tokushima

• UENO Masaharu

  Faculty of Pharmaceutical Sciences, University of Toyama

• Okuda O

  Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University

• 奥田 修

  Faculty Of Pharmaceutical Sciences Osaka University

• Ueno Masaharu

  Faculty Of Phamaceutical Sciences Toyama Medical And Pharmaceutical University

• OKUDA Otomo

  Faculty of Pharmaceutical Sciences Toyama Medical and Pharmaceutical University

• NAKAMURA Chiharu

  Faculty of Pharmaceutical Sciences Toyama Medical and Pharmaceutical University

• Hayashi K

  Graduate School Of Medicine And Pharmaceutical Sciences For Research University Of Toyama

• Ueno M

  Fac. Of Pharmaceutical Sciences Univ. Of Toyama

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