Physicochemical Properties of PEG-Grafted Liposomes
スポンサーリンク
概要
- 論文の詳細を見る
Egg phosphatidylcholine (EggPC) or dimyristoylphosphatidylcholine (DMPC) liposomes containing polyethylene glycol (PEG)-lipids covering a range of 0-30 mol% have been prepared by Extrusion method. The physicochemical properties including size evolution and calcein permeation were evaluated to investigate the effect of PEG-lipids on bilayer structure. The results from quasielasetic light scattering (QELS), freeze-fracture microscopy, and gel exclusion chromatography revealed that presence of low concentration of PEG-lipid results in decreasing of vesicle size and further increase in the PEG-lipid concentrations lead to a transition from the lamellar membranes to micelles. The permeability for calcein increased with increase in concentration of distearoylphosphatidylethanolamine (DSPE)-PEG. On the other hand, the permeability decreased with low amount of cholesterol-PEG (blow 20% cholesterol-PEG) and increased with high amount of it. The maximum concentration of PEG-lipid that may be incorporated without alteration of the liposome structure depends on the composition of the bilayer. The concentration of DSPE-PEG2000 incorporated into vesicles without damaging vesicle structures were <20 mol% for EggPC and <10% for DMPC.
- 公益社団法人日本薬学会の論文
- 2002-09-01
著者
-
Ueno Masaharu
Faculty Of Phamaceutical Sciences Toyama Medical And Pharmaceutical University
-
Sriwongsitanont Supaporn
Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
関連論文
- Process of Destruction of Large Unilamellar Vesicles by a Zwitterionic Detergent, CHAPS : Partition Behavior between Membrane and Water Phases
- Difference between Yeast and Bovine Mitochondrial ADP/ATP Carriers in Terms of Conformational Properties of the First Matrix Loop as Deduced by Use of Copper-o-phenanthroline(Molecular and Cell Biology)
- Delivery of Condensed DNA by Liposomal Non-viral Gene Delivery System into Nucleus of Dendritic Cells(Biopharmacy)
- Effects of Incorporation of Various Amphiphiles into Recipient Liposome Membraneson Inter-Membrane Protein Transfer
- Physical Properties of Phosphatidyicholine Vesicles Containing Small Amount of Sodium Cholate and Consideration on the Initial Stage of Vesicle Solubilization
- Development of a Membrane Fusible Drug Carrier from Erythrocytes by the Spontaneous Transfer of Viral Fusion Protein from Influenza Virus-Infected Cells
- Magnetic Targeting of Thermosensitive Magnetoliposomes to Mouse Livers: An In Situ On-line Perfusion System.
- Effect of Freeze-Thawing and Polyethylene Glycol (PEG) Lipid on Fusion and Fission of Phospholipid Vesicles
- Size Growth Factor in the Process of Vesicle Formation form Phospholipid-Detergent Mixed Mecelles
- Hyperfine Splitting of 2, 2, 6, 6-Tetramethylpiperidine-N-oxyl in Octyl β-D-Glucoside-Containing Large Unilamellar Vesicle Suspensions
- Size Control of Mixed Egg Yolk Phosphatidylcholine (EggPC)/Oleate Vesicles
- BASIC STUDY ON HEPATIC ARTERY CHEMOEMBOLIZATION USING TEMPERATURE-SENSITIVE LIPOSOME
- Reconsideration of Drug Release from Temperature-Sensitive Liposomes
- Physicochemical Properties of PEG-Grafted Liposomes
- Membrane Fluidity of Egg Yolk Phosphatidylcholine/Detergent Mixed Aggregates Studied by Spin Label Method
- New Vesicle Formation upon Oleate Addition to Preformed Vesicles
- 13C-NMR study of aerosol OT in chloroform.
- The behavior of aerosol OT in organic solvents.
- Characteristics of the Membrane Permeability of Temperature-Sensitive Liposome.