Physical Properties of Phosphatidyicholine Vesicles Containing Small Amount of Sodium Cholate and Consideration on the Initial Stage of Vesicle Solubilization

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概要

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• The effects of sub-solubilizing concentrations of sodium cholate (Na-chol) on several physicochemical properties of phosphatidylcholine (PC) small unilamellar vesicles (SUV) were considered in connection with the initial stage of membrane solubilization. ESR spectra of 12-doxylstearic acid (12-DS) in phosphatidylcholine from egg yolk (EPC) or dimyristoylphosphatidylcholine (DMPC) SUV at low concentrations (insufficient to destroy the vesicles) of Na-chol were composed of two (a strongly immobilized and an additional weakly immobilized) immiscible components. The origin of the additional bands was phase separation which occurred in the hydrophobic parts of PC SUV in the presence of Na-chol. Differential scanning calorimetry measurements demonstrated that the mixed DMPC/Na-chol SUV possessed two (a sharp low-temperature and a broad high-temperature) endothermic peaks, which is consistent with the coexistence of two immiscible phases in the vesicular membranes. ζ Potentials of the EPC/Na-chol SUV revealed that high anionic densities appeared on the surfaces of the SUV at a Na-chol concentration slightly below the upper boundary of the vesicle region. Thus, the initial stage of the solubilization of PC SUV by Na-chol was caused by the aggregation of hydrophobic parts of PC membranes, followed by the occurrence of high anionic densities on the surfaces of the vesicles. The fact that removal of Na-chol from PC/Na-chol mixed systems preferentially resulted in the formation of small vesicles might originate from these anionic charges.

• 公益社団法人日本薬学会の論文
• 2002-09-01

著者

• KASHIWAGI HIROSHI

  Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University

• SUN Changqi

  Faculty of Pharmaceutical Sciences, Kyoto University

• UENO Masaharu

  Faculty of Pharmaceutical Sciences, University of Toyama

• Kashiwagi Hiroshi

  Department Of Surgery Jichi Medical School

• Sun C

  Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University

• Sun Changqi

  Faculty Of Pharmaceutical Sciences Kyoto University

• Ueno Masaharu

  Faculty Of Phamaceutical Sciences Toyama Medical And Pharmaceutical University

• Kashiwagi H

  Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University

• Koiwai Hirobumi

  Department Of Gastroenterology Jichi Medical Schooldepartment Of Pathology Jichi Medical School

• Kashiwagi Hiroshi

  Faculty Of Computer Science And Systems Engineering Kyushu Institute Of Technology

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