Induction of Transition and Transversion Mutations during Random Mutagenesis PCR by the Addition of 2-Hydroxy-dATP (Biochemistry/Molecular Biology)
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概要
- 論文の詳細を見る
A 2-substituted purine nucleotide analog, 2-hydroxy-2'-deoxyadenosine 5'-triphosphate (2-OH-dATP), was added to a PCR mixture, to examine its mutagenic potential. The 2-OH-dATP enhanced the total mutation frequency. Interestingly, 2-OH-dATP induced both transition and transversion mutations, including A : T → G : C, A : T → C : G and G : C → T : A mutations. In contrast, other 2-substituted purine nucleotide analogs, 2-aminopurine-2'-deoxyriboside 5'-triphosphate and 2-amino-2'-deoxyadenosine 5'-triphosphate, did not affect the total mutation frequency. These results suggest that 2-OH-dATP is useful in random PCR mutagenesis for the in vitro evolution of nucleic acids and proteins, and for analyses of residues in these biomolecules.
- 公益社団法人日本薬学会の論文
- 2004-05-01
著者
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KAMIYA Hiroyuki
Graduate School of Pharmaceutical Sciences, Hokkaido University
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HARASHIMA Hideyoshi
Graduate School of Pharmaceutical Sciences, Hokkaido University
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Ito M
Meijo Univ. Nagoya Jpn
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Ito M
Graduate School Of Pharmaceutical Sciences Kyoto University
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Ito M
Department Of Pharmacognosy Graduate School Of Pharmaceutical Sciences Kyoto University
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Ito Michiho
北里大学 薬学部
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Harashima Hideyoshi
Department Of Pharmacokinetics And Pharmaceutics Graduate School Of Pharmaceutical Sciences The Univ
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Kamiya Hiroyuki
産業医科大学産業生態科学研究所
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Murata-kamiya Naoko
Inst. Indust. Ecolog. Sci. Univ. Occup. Environ. Hith.
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ITO Mana
Graduate School of Pharmaceutical Sciences, Hokkaido University
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Kamiya Hiroyuki
Graduate School Of Pharmaceutical Sciences Hokkaido University
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Kaji Hiroshi
Inst. Indust. Ecolog. Sci. Univ. Occup. Environ. Hith.
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Harashima Hideyoshi
Graduate School Of Pharmaceutical Sciences Hokkaido University:crest Japan Science And Technology Corporation (jst)
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